Method and apparatus for obtaining an absolute white blood cell subset count and white blood cell multipart differential
September 20, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesAutomated single-slide staining device Diagnostic method employing a mixture of normally separable protein-coated particles Novel material for immunological assay of biochemical components and a process for the determination of said components Microcapsules sensitized with antibody and a method for measurement of lymphocyte using the same based on cell-mediated immunity Simultaneous assay for T and B lymphocyte populations and subpopulations Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes Method of staining and identifying cells and compositions thereof Monoclonal antibody specific to a common determinant site of neutrophils and eosinophils Monoclonal antibody specific to neutrophils Novel 7-aroyl-4-hydroxy-3-methyl-benzofurans as dual inhibitors of cyclooxygenase and 5-lipoxygenase InventorsAssigneeApplicationNo. 703163 filed on 05/20/1991US Classes:435/7.24, Leukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)435/7.2, Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate435/7.21, Animal cell435/7.23Tumor cell or cancer cellExaminersPrimary: Wax, Robert A.Assistant: Moore, William W. Attorney, Agent or FirmForeign Patent References
International ClassesG01N 033/53G01N 033/48 G06M 011/02 AbstractAn optical screening method and apparatus for identifying and counting cells expressing selected characteristics or properties. The cells are combined with one or more different sets of microspheres, each set having a reactant bound thereto which will bind to a specific molecule which can exist on one or more types of the cells. The cells and microspheres are formed in a known volume on a slide and optically viewed to identify and count the type of cells to which the different sets of microspheres do or do not bind. The cell count then is related to the known volume to provide an absolute cell count. A plurality of different sample portions can be utilized with different reactants to obtain a multipart WBC differential/absolute count. The different sets of microspheres are optically differentiated by having different optical characteristics, such as size, shape, color or combinations thereof.Other References
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