Assay for nucleic acid sequences in an unpurified sample
October 4, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSpecific DNA probes in diagnostic microbiology Method for the direct analysis of sickle cell anemia Nucleic acid hybridization assay employing antibodies to intercalation complexes Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same Test for Salmonella Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays Solution-phase single hybridization assay for detecting polynucleotide sequences Patent #: 4968602 InventorsAssigneeApplicationNo. 772625 filed on 10/04/1991US Classes:435/6, Involving nucleic acid435/29, Involving viable micro-organism435/34, Determining presence or kind of micro-organism; use of selective media435/35, Using radioactive material435/36, Streptococcus; staphylococcus436/63, BIOLOGICAL CELLULAR MATERIAL TESTED436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Parr, MargaretAssistant: Horlick, Kenneth R. Attorney, Agent or FirmForeign Patent References
International ClassC12Q 001/68AbstractA method for detecting (i) one or more microorganisms or (ii) nucleic acid sequences from a prokaryotic source or an eukaroytic source in an unpurified nucleic acid-containing test sample comprising(a) labeling the nucleic acids in the test sample,(b) contacting, under hybridization conditions, the labeled hybridizable nucleic acid and one or more immobilized hybridizable nucleic acid probes comprising (i) one or more known microorganisms or (ii) sequences from eukaroytic or prokaryotic sources, to form hybridized labeled nucleic acids, and(d) assaying for the hybridized nucleic acids by detecting the label. The method can be used to detect genetic disorders, e.g., sickle-cell anemia.Other References
Field of SearchInvolving nucleic acidPolynucleotide (e.g., nucleic acid, oligonucleotide, etc.) Involving viable micro-organism Determining presence or kind of micro-organism; use of selective media Using radioactive material Streptococcus; staphylococcus Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA BIOLOGICAL CELLULAR MATERIAL TESTED BIOSPECIFIC LIGAND BINDING ASSAY |
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