Subtilisin modifications to enhance oxidative stability
Patent 5346823 Issued on September 13, 1994. Estimated Expiration Date: 
March 24, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
March 24, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.InventorsAssigneeApplicationNo. 036592 filed on 03/24/1993US Classes:435/222, Bacillus subtilus or bacillus lichenoformis435/23, Involving proteinase435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/221, Bacteria is bacillus435/252.31, Bacillus (e.g., B. subtilis, B. thuringiensis, etc.)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/23.2Encodes an enzymeExaminersPrimary: Wax, Robert A.Assistant: Moore, William W. Attorney, Agent or FirmInternational ClassesC12N 015/00C12N 015/57 C12N 015/75 C12N 015/11 AbstractThere are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.Other References
Field of SearchRecombinant DNA technique included in method of making a protein or polypeptideBacteria is bacillus Bacillus subtilus or bacillus lichenoformis Bacillus (e.g., B. subtilis, B. thuringiensis, etc.) VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) Encodes an enzyme |
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