Method and apparatus for optically screening microscopic cells
August 23, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesAutomated single-slide staining device Diagnostic method employing a mixture of normally separable protein-coated particles Novel material for immunological assay of biochemical components and a process for the determination of said components Bright field light microscopic method of enumerating and characterizing subtypes of white blood cells and their precursors Microcapsules sensitized with antibody and a method for measurement of lymphocyte using the same based on cell-mediated immunity Simultaneous assay for T and B lymphocyte populations and subpopulations Metachromatic dye sorption means for differential determination of sub-populations of lymphocytes Method of staining and identifying cells and compositions thereof Composition, synthesis and use of simulated cells Monoclonal antibody specific to a common determinant site of neutrophils and eosinophils InventorsApplicationNo. 617096 filed on 11/23/1990US Classes:435/7.21, Animal cell435/7.2, Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate435/7.23, Tumor cell or cancer cell435/7.24Leukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)ExaminersPrimary: Wax, Robert A.Assistant: Moore, William W. Attorney, Agent or FirmForeign Patent References
International ClassesG01N 033/50G01N 033/80 G01N 001/28 G01N 021/00 AbstractAn optical screening method and apparatus for identifying both the morphology and selected characteristics or properties expressed by cells. The cells are combined with one or more different sets of microspheres, each set having a reactant bond thereto which will bind to a specific molecule which can exist on one or more types of the cells. The cells and microspheres are formed as a smear on a slide, stained with a histological type stain and optically viewed to identify the type of cells to which the different sets of microspheres do or do not bind. The different sets of microspheres are optically differentiated by having different optical characteristics, such as size, shape, color or combinations thereof. A fast cell screening method is provided by the observation of clumping of the cells and microspheres, where present. Also, cells can be screened by utilizing a first reactant which will bind to a specific cell molecule and binding a second reactant to the first reactant, which second reactant is bound to a set of microspheres.Other References
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