Nucleic acid probe, test kit and diagnostic and purification methods
March 6, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesFine carrier particles sensitized with acylated antibody for antigen detection Process for amplifying, detecting, and/or-cloning nucleic acid sequences Coupling of nucleic acids to solid support by photochemical methods Polynucleotide determination with selectable cleavage sites Immunoreactive reagent particles having tracer, receptor molecules and protein of pI less than 6 Agglutination reagent and method of preparing same Patent #: 4828978 InventorsApplicationNo. 847447 filed on 03/06/1992US Classes:435/6, Involving nucleic acid435/5, Involving virus or bacteriophage435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Parr, MargaretAssistant: Myers, Carla J. Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C07H 021/04 AbstractAn oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques. |
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