Method and apparatus for measuring binding affinity
Patent 5324633 Issued on June 28, 1994. Estimated Expiration Date: 
November 22, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 22, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMethod for studying enzymatic and other biochemical reactions Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof Patent #: 5143854 InventorsAssigneeApplicationNo. 796947 filed on 11/22/1991US Classes:435/6, Involving nucleic acid422/52, Chemiluminescent422/82.06, Optode or optrode422/99, Miscellaneous laboratory apparatus and elements, per se435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/970, TEST STRIP OR TEST SLIDE435/973, SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE435/975, KIT436/164, OPTICAL RESULT436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/527, Glass or silica436/531, Carrier is synthetic resin436/808, Automated or kit436/809, Multifield plates or multicontainer arrays436/810, Tube, bottle, or dipstick436/823, IMMUNOGENIC CARRIER OR CARRIER PER SE530/333, Synthesis of peptides530/334Polymer supported synthesis, e.g., solid phase synthesis, Merrifield synthesis, etc.ExaminersPrimary: Kepplinger, Esther M.Assistant: Spiegel, Carol A. Attorney, Agent or FirmForeign Patent References
International ClassesG01N 033/543G01N 033/566 AbstractA method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentration. The fluorescence intensity of the labelled receptor is measured by way of, e.g., a photon counter using a confocal microscope (316). Binding affinity is determined through analysis of the relationship between fluorescence intensity and the solution concentration of the receptor. On-rates are measured as a kinetic increase in surface fluorescence intensity, and the on-rate constant rate extracted from fits to the data.Other References
Field of SearchINVOLVING KINETIC MEASUREMENT OF ANTIGEN-ANTIBODY REACTIONINVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS Glass or silica Carrier is synthetic resin Amino acid or sequencing procedure OPTICAL RESULT Spectrum analysis (e.g., flame photometry, etc.) With fluorescence or luminescence Automated or kit Multifield plates or multicontainer arrays Tube, bottle, or dipstick IMMUNOGENIC CARRIER OR CARRIER PER SE Involving nucleic acid Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay Heterogeneous or solid phase assay system (e.g., ELISA, etc.) HIGH ENERGY SUBSTRATES (E.G., FLUORESCENT, CHEMILUMINESCENT, RADIOACTIVE, ETC.) TEST STRIP OR TEST SLIDE SIMULTANEOUS DETERMINATION OF MORE THAN ONE ANALYTE KIT MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP Chemiluminescent Optode or optrode Miscellaneous laboratory apparatus and elements, per se Organic polymerization Including plural sequential reaction stages Synthesis of peptides Polymer supported synthesis, e.g., solid phase synthesis, Merrifield synthesis, etc. |
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