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Nucleic acid probes and methods for detecting fungi

Patent 5324632 Issued on June 28, 1994. Estimated Expiration Date: Icon_subject October 22, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Method for detection, identification and quantitation of non-viral organisms Patent #: 4851330
Issued on: 07/25/1989
Inventor: Kohne

Inventors

Assignee

Application

No. 780800 filed on 10/22/1991

US Classes:

435/6, Involving nucleic acid536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.32, Probes for detection of microbial nucleotide sequences536/24.33Primers

Examiners

Primary: Parr, Margaret
Assistant: Tran, Pablo N.

Attorney, Agent or Firm

Foreign Patent References

  • 0272009 EP. 06/21/1988
  • 8803957 WO. 06/21/1988

International Classes

C12C 001/68
C07H 021/04

Abstract

Nucleic acid probes are described for detecting fungi capable of causing fungal septicemia or capable of causing food spoilage. The preferred probes are complementary to ribonucleic acid sequences found in numerous fungi and absent in animal or plant genomes. As such, these probes can detect the rRNA, rDNA, or polymerase chain reaction amplification products from the majority of fungal species. The detection of etiological agents of human fungemia, the clinical diagnosis of this disease and the direct evaluation of food or beverage fungal content utilizing rRNA or rDNA probes is now possible.

Other References

  • Nucleic Acid Sequence Printout (Aug. 17, 1992), pp. 1-9
  • Matthews et al. Analytical Biochem. 169: 1-25, 1988 (Feb. 1988)
  • Cooper et al. in "Manual of Clinical Microbiology" ed. Lennette, E. H. pp. 526-541, 198
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