Abstract

A process is disclosed for regulating the size and composition of the internal reaction medium of a membrane mimetic system using clathrate hydrate formation under gas pressure conditions. When the disclosed membrane mimetic system is in contact with a clathrate forming gas, some of the water in the internal reaction medium is converted to crystalline hydrate form, which physically and chemically isolates the water from the remainder of the internal reaction medium. In one preferred process embodiment, the membrane mimetic system is used for enzymatic polymer synthesis in an organized medium to control the size and morphology of the polymer particles formed.

Other References

• Barbaric et al., "Micellar Solubilization of Biopolymers in Organic Solvents. 5. Activity and Conformation of ଱-Chymotrypsin in Isooctane-AOT Reverse Micelles," J. Am. Chem. Soc., vol. 103, pp. 4239-4244 (1981)
• Candau et al., "Inverse Microlatexes: Mechanism of Formation and Characterization," Structure and Reactivity in Reverse Micelles, pp. 361-370 (M. P. Pileni, ed. 1989)
• Hagen et al., "Protein Refolding in Reversed Micelles," Biotechnology and Bioengineering, vol. 35, pp. 955-965 (1990)
• John et al., "Pressurized Gases in Contact with Liquid Reversed Micellar Solutions: Effects on Micellar Size and Stability, Biotechnological Application," Proceedings of the Second International Symposium on Supercritical Fluids, pp. 70-73 (1991)
• Kabanov et al., "Enzymes Entrapped in Reversed Micelles of Surfactants in Organic Solvents: A Theoretical Treatment of the Catalytic Activity Regulation", J. Theor. Biol., vol. 133 pp. 327-343 (1988)
• Kortan et al., "Nucleation and Growth of CdSe on ZnS Quantum Crystallite Seeds, and Vice Versa, in Inverse Micelle Media," J. Am. Chem. Soc., vol. 112, pp. 1327-1332 (1990)
• Luisi, P. L., "Enzymes Hosted in Reverse Micelles in Hydrocarbon Solution," Angewandte Chemie International Edition in English, vol. 24, pp. 439-450 (1985)
• Nguyen et al., "Characteristics of Protein-Containing Reversed Micelles Subjected to Hydrate Formation Conditions," J. Phys. Chem. vol. 95, pp. 1467-1471 (1991)
• Nguyen et al., "Clathrate Hydrate Formation in Reversed Micelles," J. Phys. Chem., vol. 93, pp. 8123-8126 (1989)
• Phillips et al., "Protein Recovery from Reversed Micellar Solutions through Contact with a Pressurized Gas Phase," Biotechnology Progress, vol. 7, pp. 43-48 (1991)
• Rao et al., "Modification of Enzyme Activity in Reversed Micelles through Clathrate Hydrate Formation," Biotechnology Progress, vol. 6, pp. 465-471 (1990)
• Zulauf et al., "Inverted Micelles and Microemulsions in Ternary System H₂ O/Aerosol-OT/Isooctane As Studied by Photon Correlation Spectroscopy," J. Phys. Chem., vol. 83, pp. 480-486, (1979)
• Akkara et al., "Synthesis and Characterization of Polymers Produced by Horseradish Peroxidase in Dioxane," J. Polymer Sci., vol. 29, pp. 1561-1574 (1991)
• Dordick et al., "Polymerization of Phenols Catalyzed by Peroxidase in Nonaqueous Media," Biotechnol. Bioeng., vol. 30, pp. 31-36 (1987