Assays for inhibitors of leukocyte adhesion
Patent 5318890 Issued on June 7, 1994. Estimated Expiration Date: 
June 7, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 7, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.InventorsApplicationNo. 695805 filed on 05/06/1991US Classes:435/7.24, Leukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/7.2, Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)530/387.3Chimeric, mutated, or recombined hybrid (e.g., bifunctional, bispecific, rodent-human chimeric, single chain, rFv, immunoglobulin fusion protein, etc.)ExaminersPrimary: Schwartz, Richard A.Assistant: Guzo, David Attorney, Agent or FirmInternational ClassesC12Q 001/00C12Q 001/02 AbstractThe present invention provides novel assays for determining the ability of a test compound to inhibit intercellular adhesion mediated by a selectin receptor, such as the LHR. The assays involve contacting the test compound with the receptor and an isolated receptor-binding agent. The receptor-binding agent is a sulfated polysaccharide, a sulfated glycolipid, or a compound comprising the extracellular region of an endothelial cell surface glycoprotein. Also provided are compositions comprising a compound comprising the extracellular region of an endothelial cell surface protein, which is specifically recognized by lymphocyte homing receptors.Other References
Field of SearchLeukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay Involving a micro-organism or cell membrane bound antigen or cell membrane bound receptor or cell membrane bound antibody or microbial lysate Heterogeneous or solid phase assay system (e.g., ELISA, etc.) Competitive assay Binds lymphocytic or lymphocytic-like cell or component or product thereof (e.g., B cell, B-lineage bone marrow cell, null cell, natural killer cell, B-lymphoblastoid cell, B-lineage acute lymphoblastic leukemia cell, B-lymphocytic cell-surface antigen, etc.) |
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