Voltammetric sequence-selective sensor for target polynucleotide sequences
Patent 5312527 Issued on May 17, 1994. Estimated Expiration Date: 
October 6, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
October 6, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesApplication of tetrathiafulvalenes in bioelectrochemical processes Detection of AIDS associated virus by polymerase chain reaction Oligonucleotides and kits for detection of HTLVI and HTLVII viruses by hybridization Method for detection of specific nucleic acid sequences Patent #: 5185243 InventorsAssigneeApplicationNo. 957602 filed on 10/06/1992US Classes:205/777.5, Involving enzyme or micro-organism204/403.01, Biological material (e.g., microbe, enzyme, antigen, etc.) analyzed, tested, or included in apparatus204/403.15, Electrode containing free carbon204/412, Three or more electrodes435/287.2, Measuring or testing for antibody or nucleic acid, or measuring or testing using antibody or nucleic acid435/810, PACKAGED DEVICE OR KIT435/817, ENZYME OR MICROBE ELECTRODE436/94, Saccharide (e.g., DNA, etc.)436/501BIOSPECIFIC LIGAND BINDING ASSAYExaminersPrimary: Niebling, John F.Assistant: Bell, Bruce F. Attorney, Agent or FirmForeign Patent References
International ClassG01N 027/26AbstractThe present invention relates to a voltammetric sequence-selective sensor for target polynucleotide sequences which essentially comprises: an immobilized polynucleotide probe having one end covalently bound onto an amperometric electrode. The immobilized probe includes a target binding region capable of hybridizing to the target polynucleotide sequences forming an immobilized heteroduplex having at least a hybridized region. The sequence-selective sensor of the present invention also comprises means for voltammetrically detecting immobilized heteroduplexes. There is also provided a method for detecting the presence of a target polynucleotide analyte in a physiological sample, which comprises the steps of incubating the prepared physiological sample with the voltammetric sequence-selective sensor of the present invention; voltammetrically detecting immobilized heteroduplexes; and comparing the resulting voltammogram with a control voltammogram. |
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