Abstract Claims Description Full Text

Abstract

Plant cells are transformed by bringing them into contact with a a multiplicity of needle-like bodies on which the cells may be impaled. This causes a rupture in the cell wall allowing entry of transforming DNA either from a surrounding liquid medium or of DNA previously bound to or otherwise entrapped in the needle-like projections.

Other References

• Neuhaus, et al (Dec. 1987) Theor Appl Genet 75:30-36
• Neuhaus, et al (1986) The EMBO J. 5(7):1437-1444
• Potrykus (Jun. 1990) Bio/Technology 8:535-542
• Appel et al (Oct. 1988) Proc. Natl. Acad. Sci, USA 85:7670-7674
• Costanzo et al (Nov. 1988) Genetics 120: 667-670
• Fechheimer et al. (Dec. 1987) Proc. Natl. Acad. Sci, USA 84:8463-8467
• "Whiskers", The Condensed Chronical Dictionary, Seventh Edition, Ed. Arthur & Elizabeth Rose, Reinhold Publishing Corp., 1966, p. 1018
• Wu et al, "Infection and Synthesis Rate of Southern Bean Mosaic Virus in Soybean Callus Cells under Selected Cultural Conditions", Phytopathology, vol. 68, pp. 1389-1392, 1978
• Alberts et al, "Special Features of Plant Cells", Molecular Biology of the Cell, Garland Publishing Inc., 2nd Edition, 1983, p. 1099
• Armstrong et al, "Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline"Planta, 1985, vol. 164, pp. 207-214
• Kamo et al, "Establishment and characterization of long-term embryognic maize callus and cell suspension cultures", Plant Science, vol. 45, 1986, pp. 111-117
• Cockburn et al, "Simple Rapid Method for Gene Transfer", U.S. Patent Appln. 07/472,538, 1990
• Kaeppler et al, "Silicon carbide fiber-mediated DNA delivery into plant cells", Plant Cell Reports, vol. 9, pp. 415-418, 1990
• Cockburn, "Diligence and Ingenuity Pay Off in a Genetic Engineering Laboratory", 1990 Yearbook of Agriculture, pp. 114-116
• Raloff, "Needling tissues to accept foreign genes", Science News, p. 181, 1990
• Asano et al, "Electroporation-mediated and silicon carbide fiber-mediated DNA delivery in Agrostis alba L. (Redtop)", Plant Science, vol. 79, pp. 247-252, 1991
• Kaeppler et al, "Silicon carbide fiber-mediated stable transformation of plant cells", Theor Appln Genet, vol. 84, pp. 560-566, 1992
• Dunahay, "Nuclear Transformation of Chlamydomonas reinhardtii with silicon carbide fibers", J. Phycol., vol. 28, p. 11, Abstract No. 59, 1992
• Brown et al, "Not allowing the dust to settle", Chemistry in Britain, Oct. 1992, pp. 910-91