Transformation of plant cells

Patent 5302523 Issued on April 12, 1994. Estimated Expiration Date:

December 11, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Plant cell microinjection technique Patent #: 4743548
Issued on: 05/10/1988
Inventor: Crossway , et al.

Inventors

Assignee

Zeneca Limited

Application

No. 804664 filed on 12/11/1991

US Classes:

435/470Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection

Examiners

Primary: Chereskin, Che S.

Attorney, Agent or Firm

Cushman, Darby & Cushman

International Class

C12N 005/14

Foreign Application Priority Data

1989-06-21 GB

Abstract

Plant cells are transformed by bringing them into contact with a a multiplicity of needle-like bodies on which the cells may be impaled. This causes a rupture in the cell wall allowing entry of transforming DNA either from a surrounding liquid medium or of DNA previously bound to or otherwise entrapped in the needle-like projections.

Other References

Neuhaus, et al (Dec. 1987) Theor Appl Genet 75:30-36
Neuhaus, et al (1986) The EMBO J. 5(7):1437-1444
Potrykus (Jun. 1990) Bio/Technology 8:535-542
Appel et al (Oct. 1988) Proc. Natl. Acad. Sci, USA 85:7670-7674
Costanzo et al (Nov. 1988) Genetics 120: 667-670
Fechheimer et al. (Dec. 1987) Proc. Natl. Acad. Sci, USA 84:8463-8467
"Whiskers", The Condensed Chronical Dictionary, Seventh Edition, Ed. Arthur & Elizabeth Rose, Reinhold Publishing Corp., 1966, p. 1018
Wu et al, "Infection and Synthesis Rate of Southern Bean Mosaic Virus in Soybean Callus Cells under Selected Cultural Conditions", Phytopathology, vol. 68, pp. 1389-1392, 1978
Alberts et al, "Special Features of Plant Cells", Molecular Biology of the Cell, Garland Publishing Inc., 2nd Edition, 1983, p. 1099
Armstrong et al, "Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline"Planta, 1985, vol. 164, pp. 207-214
Kamo et al, "Establishment and characterization of long-term embryognic maize callus and cell suspension cultures", Plant Science, vol. 45, 1986, pp. 111-117
Cockburn et al, "Simple Rapid Method for Gene Transfer", U.S. Patent Appln. 07/472,538, 1990
Kaeppler et al, "Silicon carbide fiber-mediated DNA delivery into plant cells", Plant Cell Reports, vol. 9, pp. 415-418, 1990
Cockburn, "Diligence and Ingenuity Pay Off in a Genetic Engineering Laboratory", 1990 Yearbook of Agriculture, pp. 114-116
Raloff, "Needling tissues to accept foreign genes", Science News, p. 181, 1990
Asano et al, "Electroporation-mediated and silicon carbide fiber-mediated DNA delivery in Agrostis alba L. (Redtop)", Plant Science, vol. 79, pp. 247-252, 1991
Kaeppler et al, "Silicon carbide fiber-mediated stable transformation of plant cells", Theor Appln Genet, vol. 84, pp. 560-566, 1992
Dunahay, "Nuclear Transformation of Chlamydomonas reinhardtii with silicon carbide fibers", J. Phycol., vol. 28, p. 11, Abstract No. 59, 1992
Brown et al, "Not allowing the dust to settle", Chemistry in Britain, Oct. 1992, pp. 910-91