Parital primary amino acid sequence of the antineoplastic protein (ANUP); a cytokine present in granulocytes
Patent 5298604 Issued on March 29, 1994. Estimated Expiration Date: 
September 2, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 2, 2013. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesIsolation of an antineoplastic protein fraction and an antineoplastic peptide fraction from human urine Purified antineoplaston fractions and methods of treating neoplastic disease Deblocking amino terminal N-acetyl serine and N-acetyl threonine residues in peptides and proteins to allow sequencing Patent #: 5008372 InventorApplicationNo. 116539 filed on 09/02/1993US Classes:530/351, Lymphokines, e.g., interferons, interlukins, etc.530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES530/380Blood proteins or globulins, e.g., proteoglycans, platelet factor 4, thyroglobulin, thyroxine, etc.ExaminersPrimary: Schain, Howard E.Attorney, Agent or FirmInternational ClassC07K 015/12AbstractElectrophoretically homogeneous human Antineoplastic Urinary Protein (ANUP) contains a blocked N-terminal amino acid that has been identified as pyroglutamic acid. Removal of the pyroglutamy residue by the use of pyroglutamyl aminopeptidose results in the formation of the deblocked protein which is also an antineoplastic molecule. The amino acid sequence of the deblocked ANUP 16 KD monomer showed the following sequence:Cycle No.1. Leu L2. Lys K3. Cys C4. Tyr Y5. Thr T6. Cys C7. Lys K8. Glu E9. Pro P10. Met MCycle No.11. Thr T12. Thr (T)? or Ser (S)?13. Ala A14. Ala A15. X?A data base search using the above sequence showed that 100% homology with another protein was not found regardless of unassigned positions.The blocked N-terminal amino acid of ANUP is pyroglutamic acid.Other References
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