Heterofunctional crosslinking agent for immobilizing reagents on plastic substrates
March 1, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesPreparation of trichloro-s-triazine activated supports for coupling ligands Preactivated plastics surfaces for immobilizing organo-chemical and biologic materials Protein immobilization by adsorption of a hydrophobic amidine protein derivative to a hydrophobic surface Protein label and drug delivery system Covalent attachment of antibodies and antigens to solid phases using extended length heterobifunctional coupling agents Patent #: 5002883 InventorsApplicationNo. 663120 filed on 03/01/1991US Classes:435/181, Attached to the carrier via a bridging agent435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)436/532, Antigen or antibody attached to a carrier via bridging agent530/816, Attached to the carrier via a bridging agent560/190Polycarboxylic acidExaminersPrimary: Naff, David M.Attorney, Agent or FirmForeign Patent References
International ClassesC12N 011/06G01N 033/549 C07C 069/34 AbstractHeterofunctional crosslinking agents are synthesized that covalently link molecules such as enzymes, cells, proteins and nucleic acids to a plastic substrate. The agents contain a central ring structure having a hydrophobic hydrocarbon chain that binds to a plastic substrate and distal to the hydrophobic chain one or more hydrophilic chains terminating in a reactive group that covalently binds the molecule. Immobilized molecules are useful in diagnostic assays or bioreactors. A preferred heterofunctional crosslinking agent is succinyl-olivetol-N-hydroxysuccinimide having the structure: ##STR1## This agent is prepared by reacting succinic anhydride with 5-pentyl resorcinol and condensing carboxylic acid groups with N-hydroxysuccinimide. |
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