Method and kit for performing nucleic acid hybridization assays
November 20, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesFluorescent scavenger particle immunoassay Specific DNA probes in diagnostic microbiology Immunometric assays using monoclonal antibodies Process for determining tumor-associated glycolinkage Process for applying multiple layers of a protein and a ligand extender to a surface and to the multiple-layer system Detection of microbial nucleic acids by a one-step sandwich hybridization test Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays Assay method and probe for polynucleotide sequences Nucleic acid hybridization assay employing antibodies to intercalation complexes Method of detecting and characterizing a nucleic acid or reactant for the application of this method InventorsAssigneeApplicationNo. 798027 filed on 11/20/1991US Classes:435/6, Involving nucleic acid435/5, Involving virus or bacteriophage536/24.3Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNAExaminersPrimary: Moskowitz, MargaretAssistant: Chambers, Scott A. Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/70C12Q 001/68 C07H 021/04 AbstractA method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.Other References
|
| ||||||||||||||
