Method for preparing transformed plant

Patent 5272072 Issued on December 21, 1993. Estimated Expiration Date:

October 30, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Plant generation method Patent #: 4840906
Issued on: 06/20/1989
Inventor: Hunter

Inventors

Assignee

Sapporo Breweries Limited

Application

No. 784709 filed on 10/30/1991

US Classes:

800/276, METHOD OF CHEMICALLY, RADIOLOGICALLY, OR SPONTANEOUSLY MUTATING A PLANT OR PLANT PART WITHOUT INSERTING FOREIGN GENETIC MATERIAL THEREIN435/460, Involving laser treatment of the cell before or during transfection800/320Gramineae (e.g., barley, oats, rye, sorghum, millet, etc.)

Examiners

Primary: Fox, David T.
Assistant: Rories, Charles C. P.

Attorney, Agent or Firm

Oblon, Spivak, McClelland, Maier & Neustadt

Foreign Patent References

0270356 EP. 06/13/1988
0275069 EP. 07/13/1988
0290395 EP. 11/13/1988
0334539 EP. 09/13/1989
WO89/00602 WO. 01/13/1989

International Classes

C12N 015/12
C12N 005/00
A01H 001/04

Foreign Application Priority Data

1990-11-01 JP

Abstract

A transformed plant of Gramineae is prepared by culturing an anther of Gramineae in a callus induction medium and, at a stage immediately before the microspore begins to cause division or during the initial division, transducing a genetic substance into the microspore cell through a pore formed by a laser pulse thereby to express genetic information of the genetic substance.According to the present invention, it is unnecessary to prepare protoplast and therefore, time and operations for transformation can be greatly reduced. Since haploid cells are transformed, the character transduced is conveyed without separating at a later generation. In addition, difficulties in experiments between species and strains are minimized so that it is easy to apply the present invention to practical species. According to the present invention, large pores can be formed as compared to the electroporation method so that DNA or substances having a large molecular weight can be introduced.

Other References

Finn Lok Olsen, "Induction of Microspore Embryogenesis in Cultured Anthers of Hordeum Vulgare. The Effects of Ammonium Nitrate, Glutamine and Asparagine as Nitrogen Sources", Carlsberg Res. Commun., vol. 52, pp. 393-404 (1987)
K. J. Kasha et al., "Haploids in Cereal Improvement: Anther and Microspore Culture", Gene Manipulation in Plant Improvement II, Plenum Press, New York, pp. 213-235 (1990)
D. Clapham et al., "Haploid Hordeum Plants from Anthers in Vitro", Z. Pflanzenzuchtg, 69, pp. 142-155 (1973)
Michael W. Lassner et al., "Simultaneous Amplification of Multiple DNA Fragments by Polymerase Chain Reaction in the Analysis of Transgenic Plants and Their Progeny", Plant Molecular Biology Reporter, vol. 7(2), pp. 116-128 (1989)
A. Ziauddin et al., "Improved Plant Regeneration from Shed Microspore Culture in Barley (Hordeum vulgare L.) CV. Igri", Plant Cell Reports, vol. 9, pp. 69-72 (1990)
B. Foroughi-Wehr et al., "Plant Production from Cultured Anthers of Hordeum vulgare L.", Z. Pflanzenzuchtg., vol. 77, pp. 198-204 (1976)
Randall K. Saiki et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", Science, vol. 239, pp. 487-491 (Jan. 29, 1988)
Ingo Potrykus, "Gene Transfer to Cereals: An Assessment", Biotechnology, pp. 535-542 (Jun. 1990)
Weber, G., et al., Plant Cell, Tissue, and Organ Culture, vol. 12, (1988), pp. 219-22