Plant promoter -glucuronidase gene construct
Patent 5268463 Issued on December 7, 1993. Estimated Expiration Date: 
December 7, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
December 7, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesMarker genes in pseudomonad bacteria Patent #: 4753876 InventorApplicationNo. 447976 filed on 12/08/1989US Classes:536/23.7, Encodes a microbial polypeptide435/200, Acting on glycosyl compound (3.2)435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)536/24.1Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)ExaminersPrimary: Schwartz, Richard A.Assistant: LeGuyader, John L. Attorney, Agent or FirmForeign Patent References
International ClassesC12N 015/56C12N 015/82 Foreign Application Priority Data1986-11-11 GBAbstractThe present invention relates to the ଲ-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the ଲ-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the ଲ-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active ଲ-glucuronidase enzyme. Because of the abundance and availability of useful substrates for ଲ-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to ଲ-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.Other References
Field of SearchActing on glycosyl compound (3.2)Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) Encodes a microbial polypeptide Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.) |
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