Suppression of Gliocladium virens phytotoxin production with steroid inhibitors
Patent 5268173 Issued on December 7, 1993. Estimated Expiration Date: June 19, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
A process for producing cultures of Gliocladium virens for use as biocontrol agents wherein the production of viridiol is inhibited. Cells of Gliocladium virens are grown in a culture medium and under conditions effective to produce gliotoxin and/or gliovirin, wherein the culture medium includes an amount of a fungicidal steroid inhibitor which is effective to inhibit production of viridiol but which does not substantially inhibit the growth of G. virens. Following recovery, the resultant culture may be used as a biocontrol agent for the control of plant diseases by application to the locus of a plant, seedling or seed to be protected. Coating seeds with the biocontrol agent prior to planting has been found to be particularly effective for the control of soilborne root or seedling diseases.
Other References
Howell, C. R. et al. Can. J. Microbiol. 29(3), pp. 321-324, 1983
Howell, Charles R., "Biological Control of Pythium Damping-Off by Coating Cottonseed with Gliocladium viren Preparations", Beltwide Cotton Production Research Conferences, Jan. 9-14, 1990, Las Vegas, Nevada, p. 31
Howell, C. R., "Biological Control of Pythium Damping-Off of Cotton With Seed-Coating Preparations of Gliocladium virens", Phytophathology, An International Journal. vol. 81, No. 7, Jul. 1991, pp. 738-741
Howell, C. R. et al., "Phytotoxicity to Crop Plants and Herbicidal Effects on Weeds of Viridiol Produced by Gliocladium virens", vol. 74, No. 11, 1984, pp. 1346-1349, Phytopathology
Jones, Richard W., et al., "Plant Growth Response to the Phytotoxin Viridiol Produced by the Fungus Gliocladium virens", Weed Science, Issue 5, (Sep.) 1988, vol. 36:683-687
Jones, Richard W., et al., "Conversion to Viridin to Viridiol by Viridin-producing Fungi", Can. J. Microbiol., vol. 33, 1987, pp. 963-966
Howell, C. R., et al., "Antibiotic Production by Gliocladium virens and Its Relation to the Biocontrol of Seedling Diseases", Petria, Giornale di Patologia delle Piante, vol. 1(2), maggio 1991, 79-156, pp. 129-130
Howell, Charles R., "Problems and Progress in Development of Fungi as Biological Control Agents of Plant Diseases", Abstracts, 8th NACOM Innovation & Hierarchical Integration, Jackson, Wyoming, Sep. 5-8, 1990, p. 144
Howell, C. R., et al., "Antibiotic Production by Gliocladium virens and Its Relation to the Biocontrol of Cotton Seedling Diseases", 1991 Proceedings Beltwide Cotton Conference, p. 183
Howell, C. R., "Biological Control of Cotton Seedling Damping-Off By Coating Cottonseed With Gliocladium virens Preparations", Phytopathology, An International Journal, vol. 80, No. 10, Oct. 199