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Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants

Patent 5254799 Issued on October 19, 1993. Estimated Expiration Date: Icon_subject October 19, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventors

Assignee

Application

No. 555828 filed on 07/23/1990

US Classes:

800/302, Insect resistant plant which is transgenic or mutant435/418Plant cell or cell line, per se, is pest or herbicide resistant or pest lethal

Examiners

Primary: Chereskin, Che S.

Attorney, Agent or Firm

Foreign Patent References

  • 0130047 EP. 01/13/1985
  • 0142924 EP 05/13/1985
  • 0145338 EP. 06/13/1985
  • 0153166 EP. 08/13/1985
  • 0193259 EP 09/13/1986

International Classes

A01H 004/00
C12N 005/10

Abstract

Novel transformation vectors containing novel chimeric genes allow the introduction of exogenous DNA fragments coding for polypeptide toxins produced by Bacillus thuringiensis or having substantial sequence homology to a gene coding for a polypeptide toxin as described herein and expression of the chimeric gene in plant cells and their progeny after integration into the plant cell genome. Transformed plant cells and their progeny exhibit stably inherited polypeptide toxin expression useful for protecting said plant cells and their progeny against certain insect pests and in controlling said insect pests.

Other References

  • Fischhoff et al., (1987), Bio/Technology, 5:807-813
  • Fraley et al., (Aug. 1983), Proc. Natl. Acad. Sci., 80:4803-4807
  • Reiss et al., (1984), EMBO Journal, 3(13):3317-3322
  • Thorne et al., (1986), Journal of Bacteriology, 166(13):801-811
  • Hofte et al., (Jun. 1989), Microbiological Reviews, 53(2):242-255
  • Vaeck et al., (1987), Nature, 328:33-37
  • Goodman et al., (1987), Science, 236:48-54
  • Thomashow et al., "Integration and Organization of Ti Plasmid Sequences in Crown Gall Tumors", Cell, vol. 19, pp. 729-739 (1980)
  • Barnes, W. M., "A Bifunctional Gene for Insecticide and Kanamycin Resistance," Abstracts of First International Congress on Plant Molecular Biology; Savannah, Ga., USA; Oct. 1985, Abst. No. OR-21-10
  • De Block et al., "Chloroplast Transformation by Agrobacterium tumefaciens", The EMBO Journal, vol. 4, No. 6, pp. 1367-1372 (1985)
  • Jones et al., "High Level Expression of Introduced Chimaeric Genes in Regenerated Transformed Plants," The EMBO Journal, vol. 4, No. 10, pp. 2411-2418 (1985)
  • Adang et al., "Characterized Full-Length and Truncated Plasmid Clones of the Crystal Protein of Bacillus thuringiensis Subsp. Kurstaki HD-73 and Their Toxicity to Manduca sexta," Gene, vol. 36, pp. 289-300 (1985)
  • Schnepf, H. E. and Whiteley, H. R., "Delineation of a Toxin-encoding Segment of a Bacillus thuringiensis Crystal Protein Gene," The Journal of Biological Chemistry, vol. 260, No. 10, pp. 6273-6280 (1985)
  • Klier et al., "Cloning and Expression of the Crystal Protein Gene from Bacillus thuringiensis Strain Berliner 1715", The EMBO Journal, vol. 1, No. 7, pp. 791-799 (1982)
  • Hooykaas-Van Slogteren et al., "Expression of Ti Plasmid Genes in Monocotyledonous Plants Infected with Agrobacterium tumefaciens", Nature, vol. 311, No. 25, pp. 763-764 (1984
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