Transformation vectors allowing expression of Bacillus thuringiensis endotoxins in plants
Patent 5254799 Issued on October 19, 1993. Estimated Expiration Date: October 19, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
Novel transformation vectors containing novel chimeric genes allow the introduction of exogenous DNA fragments coding for polypeptide toxins produced by Bacillus thuringiensis or having substantial sequence homology to a gene coding for a polypeptide toxin as described herein and expression of the chimeric gene in plant cells and their progeny after integration into the plant cell genome. Transformed plant cells and their progeny exhibit stably inherited polypeptide toxin expression useful for protecting said plant cells and their progeny against certain insect pests and in controlling said insect pests.
Other References
Fischhoff et al., (1987), Bio/Technology, 5:807-813
Fraley et al., (Aug. 1983), Proc. Natl. Acad. Sci., 80:4803-4807
Reiss et al., (1984), EMBO Journal, 3(13):3317-3322
Thorne et al., (1986), Journal of Bacteriology, 166(13):801-811
Hofte et al., (Jun. 1989), Microbiological Reviews, 53(2):242-255
Vaeck et al., (1987), Nature, 328:33-37
Goodman et al., (1987), Science, 236:48-54
Thomashow et al., "Integration and Organization of Ti Plasmid Sequences in Crown Gall Tumors", Cell, vol. 19, pp. 729-739 (1980)
Barnes, W. M., "A Bifunctional Gene for Insecticide and Kanamycin Resistance," Abstracts of First International Congress on Plant Molecular Biology; Savannah, Ga., USA; Oct. 1985, Abst. No. OR-21-10
De Block et al., "Chloroplast Transformation by Agrobacterium tumefaciens", The EMBO Journal, vol. 4, No. 6, pp. 1367-1372 (1985)
Jones et al., "High Level Expression of Introduced Chimaeric Genes in Regenerated Transformed Plants," The EMBO Journal, vol. 4, No. 10, pp. 2411-2418 (1985)
Adang et al., "Characterized Full-Length and Truncated Plasmid Clones of the Crystal Protein of Bacillus thuringiensis Subsp. Kurstaki HD-73 and Their Toxicity to Manduca sexta," Gene, vol. 36, pp. 289-300 (1985)
Schnepf, H. E. and Whiteley, H. R., "Delineation of a Toxin-encoding Segment of a Bacillus thuringiensis Crystal Protein Gene," The Journal of Biological Chemistry, vol. 260, No. 10, pp. 6273-6280 (1985)
Klier et al., "Cloning and Expression of the Crystal Protein Gene from Bacillus thuringiensis Strain Berliner 1715", The EMBO Journal, vol. 1, No. 7, pp. 791-799 (1982)
Hooykaas-Van Slogteren et al., "Expression of Ti Plasmid Genes in Monocotyledonous Plants Infected with Agrobacterium tumefaciens", Nature, vol. 311, No. 25, pp. 763-764 (1984