Method for expression of bovine growth hormone
Patent 5254463 Issued on October 19, 1993. Estimated Expiration Date: 
October 19, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
October 19, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3664925 Recombinant DNA cloning vehicle Process for preparing bovine growth hormone Patent #: 4443539 InventorsApplicationNo. 582590 filed on 09/12/1990US Classes:435/69.4, Hormones and fragments thereof435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/252.33, Escherichia (e.g., E. coli, etc.)435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)ExaminersPrimary: Martinell, JamesAttorney, Agent or FirmForeign Patent References
International ClassesC12P 021/02C12N 015/71 C12N 015/70 C12N 015/03 AbstractMicrobial expression of an exogenous polypeptide may be inefficient if the messenger RNA has secondary structure (due to complementarity of spaced regions of the molecule) which impedes interaction with a ribosome. The occurrence of such structure can be predicted. The degeneracy of the genetic code gives freedom to alter the nucleic acid composition of the CDNA to eliminate harmful secondary structure of the MRNA.For example, bovine growth hormone (BGH) is poorly expressed by E. coli transformed with a recombinant plasmid containing the natural BGH gene. The corresponding MRNA has complementary regions at 46-51 and 73-78. Therefore a plasmid PBGH 33-4 was constructed containing a BGH gene whose upstream portion was synthesized (making use of plasmid PHGH 207-1 which contains an efficiently expressible gene for the closely related human growth hormone) so as not to cause the troublesome complementarity. E. coli transformed therewith produced BGH with-a yield of better than 105 copies per cell.Other References
Field of SearchBlood (lymphoid) cell cultureHormones and fragments thereof Interferons Signal sequence (e.g., beta-galactosidase, etc.) Antigens Lymphokines or monokines Animal tissue cell culture Interleukins Producing monoclonal antibody Fusion proteins or polypeptides Procaryotic micro-organism Using a micro-organism to make a protein or polypeptide Using tissue cell culture to make a protein or polypeptide Yeast derived Producing interferons Fused or hybrid cells Recombinant DNA technique included in method of making a protein or polypeptide Antibiotic or toxin Blood proteins Enzyme inhibitors or activators Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.) Bacillus (e.g., B. subtilis, B. thuringiensis, etc.) Brevibacterium or corynebacterium Escherichia (e.g., E. coli, etc.) Pseudomonas Streptomyces VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) |
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