Nucleic acid assay method
January 2, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3652761 Test article including a covalently attached diagnostic reagent and method Fluorescent immunoassay employing optical fiber in capillary tube Nucleic acid hybridization assay employing antibodies to intercalation complexes Immediate ligand detection assay Process for labeling nucleic acids using psoralen derivatives Immunoassay apparatus Selective zeolitic adsorbent and a method for activation thereof Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization Patent #: 4724202 InventorAssigneeApplicationNo. 817942 filed on 01/02/1992US Classes:435/6, Involving nucleic acid435/5, Involving virus or bacteriophage436/63, BIOLOGICAL CELLULAR MATERIAL TESTED436/94Saccharide (e.g., DNA, etc.)ExaminersPrimary: Chambers, Scott A.Foreign Patent References
International ClassesC12Q 001/68C12Q 001/70 G01N 033/00 G01N 033/48 AbstractAn assay for polynucleotides employing total internal reflection of excitation radiation at a coating bonded to the surface of an optically conductive glass cell. The coating initially includes single-stranded polynucleotides coupled to individual attachment sites on the surface of the cell, such polynucleotides being complementary, at least in part to the single-stranded form of the polynucleotide that is being assayed. Each molecule of the coupled polynucleotide is connected to the cell surface through a spacer connected to an irreversibly conjugated polyadenine/polythymidine sequence at one end of the coupled polynucleotide.When the surface of the coated cell is contacted with a sample that contains single-stranded polynucleotide complementary to the bound polynucleotide, renaturation will occur, forming a double-stranded form of the polynucleotide of interest. A fluorochrome dye specific to that double-stranded form is coupled to the latter, the dye including a chromophore that the excitation radiation will excite into fluorescence. The induced fluorescence is then gathered and measured.Other References
Field of SearchInvolving nucleic acid |
| ||||||||||||||
