Method for producing a biological reagent
Immunological determination using lectin
Process for the production of carrier particles from yeast cells and for producing diagnostic agents therefrom and test kits containing such agents
Cytotoxic products formed by covalent bonding of the A chain of ricin with an antibody and the process for their preparation and use
Anti Thy 1.2 monoclonal antibody-ricin hybrid utilized as a tumor suppressant
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Monoclonal antibody-ricin hybrids as a treatment of murine graft-versus-host disease
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Soluble immunoassay reagent comprising lectin covalently bonded to reactive component
Human melanoma specific immunotoxins
ApplicationNo. 560948 filed on 07/31/1990
US Classes:530/396, Lectins, e.g., concanavalin, hemagglutinin, etc.424/179.1, Conjugated via claimed linking group, bond, chelating agent, or coupling agent (e.g., conjugated to proteinaceous toxin via claimed linking group, bond, coupling agent, etc.)424/183.1, Conjugated to proteinaceous toxin or fragment thereof (e.g., conjugated to diphtheria toxin, Pseudomonas exotoxin, ricin, gelonin, abrin, etc.)530/370, Plant proteins, e.g., derived from legumes, algae or lichens, etc.530/391.7, Conjugated to a cytotoxic agent, drug, or other biologically-active substance530/402, Chemical modification or the reaction product thereof, e.g., covalent attachment or coupling, etc.530/408, Sulfur containing reactant530/409Nitrogen containing reactant
ExaminersPrimary: Wax, Robert A.
Assistant: Walsh, Stephen
Attorney, Agent or Firm
Foreign Patent References
International ClassesC07K 009/00
AbstractAn activated affinity ligand is described comprising: a ligand having (a) a region with affinity for binding sites of a lectin; and (b) a reactive group capable of covalently linking the ligand to the lectin to thereby block one or more of the binding sites of the lectin. A blocked lectin is described comprising one or more affinity ligands covalently linked by means of a reactive group present on each of the ligands to a lectin such that one or more binding sites of the lectin is blocked. A cell-binding agent-blocked lectin conjugate is described comprising the above-described blocked lectin and a cell-binding agent covalently linked to: (a) one of the covalently linked affinity ligands; or (b) the lectin. A method of preparing a blocked lectin is described. A method of preparing the cell-binding agent-blocked lectin conjugate is described. An affinity support capable of binding to a lectin to form a blocked lectin is described comprising an activated affinity ligand covalently linked to a solid support. A method of preparing the affinity support capable of binding to a lectin to form a blocked lectin is described. A method of killing selected cell populations having reduced cytotoxicity to non-selected cell populations is described comprising contacting a cell population or tissue suspected of containing cells from said selected cell population with the above-described cell-binding agent-blocked lectin conjugate, wherein the lectin is a cytotoxic lectin. Medicaments and methods of treatment using the above-described cell-binding agent-blocked lectin conjugate also are described.
Field of SearchGlycoprotein (carbohydrate containing)
Plant proteins, e.g., derived from legumes, algae or lichens, etc.
Glycoprotein, e.g., mucins proteoglycans, etc.
Lectins, e.g., concanavalin, hemagglutinin, etc.
Oxygen containing is saccharide
Chemical modification or the reaction product thereof, e.g., covalent attachment or coupling, etc.
Sulfur containing reactant
Nitrogen containing reactant
Binds receptor (e.g., transferrin receptor, Fc receptor, dihydropyridine receptor, IL-2 receptor, etc.)
Conjugated to a cytotoxic agent, drug, or other biologically-active substance