Sensitivity and selectivity of ion channel biosensor membranes

Patent 5234566 Issued on August 10, 1993. Estimated Expiration Date:

April 18, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Chemo-receptive lipid based membrane transducers
Patent #: 4661235
Issued on: 04/28/1987
Inventor: Krull , et al.

Ion selective electrode and flow type ion sensor using the same
Patent #: 4758325
Issued on: 07/19/1988
Inventor: Kanno , et al.

Chemical selective sensors utilizing admittance modulated membranes Patent #: 4776944
Issued on: 10/11/1988
Inventor: Janata , et al.

Inventors

Assignee

Australian Membrane and Biotechnology Research Institute

Application

No. 654635 filed on 04/18/1991

US Classes:

204/403.06, With semipermeable membrane204/403.08, Lipid included in apparatus204/416, Ion-sensitive electrode204/418, Organic membrane204/426, Planar electrode surface257/253, Chemical (e.g., ISFET, CHEMFET)257/414, RESPONSIVE TO NON-ELECTRICAL SIGNAL (E.G., CHEMICAL, STRESS, LIGHT, OR MAGNETIC FIELD SENSORS)435/817, ENZYME OR MICROBE ELECTRODE436/806ELECTRICAL PROPERTY OR MAGNETIC PROPERTY

Examiners

Primary: Niebling, John F.
Assistant: Bell, Bruce F.

Attorney, Agent or Firm

Spensley Horn Jubas & Lubitz

Foreign Patent References

4012385 AU. 03/13/1984
0138150 EP. 10/13/1984
2195450 GB. 09/13/1987

International Class

G01N 027/26

Foreign Application Priority Data

1988-08-18 AU

Abstract

The present invention provides a biosensor comprising at least one lipid membrane, each membrane including at least one gated ion channel. The membranes comprise a closely packed array of self-assembly amphophilic molecules and the gated ion channel has a conductance which is dependent upon an electric field applied across the membrane. The biosensor of the present invention may comprise a plurality of discrete membranes each including at least one gated ion channel. The conductance of each of the membranes is measurable independently of the conductance of the other membranes.

Other References

"Ion-Channel Sensors", Anal. Chem., Masao Sugawara, et al., 1987, 59, pp. 42-2848
"Chemical Modification of the Bilayer Lipid Membrane Biosensor Dipolar Potential" Bioelectrochem. and Bioenergetics, U. J. Krull, et al., 1986, 15, pp. 371-382
"Voltammetric Studies of Electron-Conducting Modified Bilayer Lipid Membranes" Bioelectrochem. and Bioenergetics, Pawel Drysinski, et al., 1986, 16, pp. 185-191
"Multisensing Ion-Selective Field-Effect Transistors Prepared by Ionophore Doping Technique", Anal. Chem., Klara Bezegh, et al.,. 1987, 59, pp. 2846-2848
"Chemical Derivatization of Microelectride Arrays by Oxidation of Pyrrole and N-Methylpyrrole: Fabrication of Molecule-Based Electronic Devices", J. Am. Chem. Soc., Gregg P. Kittlesen, et al., 1984, 106, pp. 7389-7396
"Dynamic and Steady-State Response of Electrochemical Detectors Based on Arrays of Small Electrodes", Anal. Chem., L. Joseph Magee, Jr., et al., 1990, 62, pp. 2625-2631
"Time and Spatial Dependence of the Concentration of Less Than 10⁵ Microelectrode-Generated Molecules", Science, Stuart Light, et al., vol. 243, pp. 1176-117