Nucleic acid amplification employing ligatable hairpin probe and transcription
Patent 5215899 Issued on June 1, 1993. Estimated Expiration Date: 
August 23, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
August 23, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesProcess for amplifying, detecting, and/or-cloning nucleic acid sequences Process for amplifying nucleic acid sequences Patent #: 4683202 InventorAssigneeApplicationNo. 569991 filed on 08/23/1990US Classes:435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/91.21, Involving the making of multiple RNA copies435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/805, TEST PAPERS436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/508, Antinuclear (e.g., DNA, etc.)436/538, Separation of immune complex from unbound antigen or antibody436/815, TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.31, Probes for detection of animal nucleotide sequences536/24.32Probes for detection of microbial nucleotide sequencesExaminersPrimary: Yarbrough, Amelia B.Assistant: Marschel, Ardin H. Attorney, Agent or FirmForeign Patent References
International ClassesC12P 019/34C12Q 001/68 AbstractSpecific nucleic acid sequences are amplified through the use of a hairpin probe which, upon hybridization with and ligation to, a target sequence is capable of being transcribed. The probe comprises a single stranded self-complementary sequence which, under hybridizing conditions, forms a hairpin structure having a functional promoter region, and further comprises a single stranded probe sequence extending from the 3' end of the hairpin sequence. Upon hybridization with a target sequence complementary to the probe sequence and ligation of the 3' end of the hybridized target sequence to the 5' end of the hairpin probe, the target sequence is rendered transcribable in the presence of a suitable RNA polymerase and appropriate ribonucleoside triphosphate (rNTPs). Amplification is accomplished by hybridizing the desired target nucleic acid sequence with the probe, ligating the target sequence to the probe, adding the RNA polymerase and rNTPs to the separated hybrids, and allowing transcription to proceed until a desired amount of RNA transcription product has accumulated. The amplification method is particularly useful in assays for the detection of particular nucleic acid sequences.Other References
Field of SearchInvolving nucleic acidTEST PAPERS VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.) BIOSPECIFIC LIGAND BINDING ASSAY Antinuclear (e.g., DNA, etc.) Separation of immune complex from unbound antigen or antibody TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS |
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