Polymer particles containing colloidal iron oxide granules for use as a magnetically responsive reagent carrier
Patent 5206159 Issued on April 27, 1993. Estimated Expiration Date: 
August 25, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
August 25, 2012. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSolid-phase immunoassays using magnetic glass Magnetic polymer particles Developer composition containing superparamagnetic polymers Magnetic polymer particles and process for the preparation thereof Process for preparing magnetic polymer particles Patent #: 4774265 InventorsAssigneeApplicationNo. 934287 filed on 08/25/1992US Classes:435/180, Carrier is synthetic polymer252/62.54, Synthetic resin252/62.56, Iron-oxygen compound containing435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)435/174, CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF435/176, Enzyme or microbial cell is immobilized on or in an inorganic carrier436/531, Carrier is synthetic resin436/534Carrier is water suspendible particlesExaminersPrimary: Naff, David M.Attorney, Agent or FirmInternational ClassesC12N 011/08C12Q 001/00 G01N 033/53 G01N 033/545 AbstractSubstantially spherical polymer particles containing a colloidally, stably and uniformly dispersed magnetically responsive substance of about 5 nm to about 500 nm in diameter provide a superparamagnetic reagent carrier. Preferably, the polymer particles have a hydrated diameter of about 0.5 to 100 um, the polymer is a non-ionic cross-linked polyacrylamide gel and the magnetically responsive substance is iron oxide granules. The magnetically responsive substance may be present in an amount so that the polymer particles have a specific gravity of between 1.2 and 2.7. The reagent carrier is prepared by swelling polymer particles in a solution of iron salt so that substantially all of the iron salt solution is taken upon by the polymer particles and then converting the iron salt in situ to insoluble colloidally dispersed iron oxide granules. Converting of the iron salt may be carried out by reacting the iron salts with a base to form iron hydroxide and subsequently converting the hydroxide by heating to iron oxide. The polymer particles are particularly useful as a reagent carrier in automated immunoassays. Enzymes, antibodies, antigens or haptens may e coupled to the polymer particles.Other References
Field of SearchCARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOFEnzyme or microbial cell is immobilized on or in an inorganic carrier Carrier is synthetic polymer MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP Heterogeneous or solid phase assay system (e.g., ELISA, etc.) Magnetic Carrier is synthetic resin Antigen or antibody attached to a carrier via bridging agent Carrier is water suspendible particles (e.g., latex, etc.) Carrier is water suspendible particles Synthetic resin Iron-oxygen compound containing |
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