Method of sequencing of genomes by hybridization of oligonucleotide probes
Patent 5202231 Issued on April 13, 1993. Estimated Expiration Date: 
June 18, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
June 18, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDiagnostic test for hepatitis B virus Hybridization assay and kit therefor Process for amplifying nucleic acid sequences Human papillomavirus 43 nucleic acid hybridization probes and methods for employing the same Patent #: 4849334 InventorsApplicationNo. 723712 filed on 06/18/1991US Classes:435/6, Involving nucleic acid436/94, Saccharide (e.g., DNA, etc.)436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.32Probes for detection of microbial nucleotide sequencesExaminersPrimary: Moskowitz, MargaretAssistant: Zitomer, Stephanie W. Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C07H 015/12 G01N 033/566 G01N 033/48 Foreign Application Priority Data1987-04-01 YUAbstractThe conditions under which oligonucleotides hybridize only with entirely homologous sequences are recognized. The sequence of a given DNA fragment is read by the hybridization and assembly of positively hybridizing probes through overlapping portions. By simultaneous hybridization of DNA molecules applied as dots and bound onto a filter, representing single-stranded phage vector with the cloned insert, with about 50,000 to 100,000 groups of probes, the main type of which is (A,T,C,G)(A,T,C,G)N8(A,T,C,G), information for computer determination of a sequence of DNA having the complexity of a mammalian genome are obtained in one step. To obtain a maximally completed sequence, three libraries cloned into the phage vector, M13, bacteriophage are used: with the 0.5 kb and 7 kbp insert consisting of two sequences, with the average distance in genomic DNA of 100 kbp. For a million bp of genomic DNA, 25,000 subclones of the 0.5 kbp are required as well as 700 subclones 7 kb long and 170 jumping subclones. Subclones of 0.5 kb are applied on a filter in groups of 20 each, so that the total number of samples is 2,120 per million bp. The process can be easily and entirely robotized for factory reading of complex genomic fragments or DNA molecules.Other References
|
| ||||||||||||||
