Polynucleotide capture assay employing in vitro amplification
April 6, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDetection of microbial nucleic acids by a one-step sandwich hybridization test Detection of microbial nucleic acids by a one-step sandwich hybridization test Process for amplifying, detecting, and/or-cloning nucleic acid sequences Process for amplifying nucleic acid sequences Reagent polynucleotide complex with multiple target binding regions, and kit and methods Displacement polynucleotide assay method and polynucleotide complex reagent therefor Displacement polynucleotide assay employing polyether and diagnostic kit Homogeneous polynucleotide displacement assay method kit and reagent complex Method and kit involving displacement and rehybridization of labeled polynucleotide Solution phase nucleic acid sandwich assay InventorAssigneeApplicationNo. 497938 filed on 03/23/1990US Classes:435/6, Involving nucleic acid435/91.2, Acellular exponential or geometric amplification (e.g., PCR, etc.)435/810, PACKAGED DEVICE OR KIT436/94, Saccharide (e.g., DNA, etc.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.33PrimersExaminersPrimary: Wax, Robert A.Assistant: Escallon, Miguel Attorney, Agent or FirmForeign Patent References
International ClassesC12Q 001/68C07H 015/12 AbstractAn analyte polynucleotide strand having an analyte sequence is detected within a sample containing polynucleotides by contacting the analyte polynucleotide with a capture probe under hybridization conditions, where the capture probe has a first binding partner specific for a solid-phase second binding partner. The resulting duplex is then immobilized by specific binding between the binding partners, and non-bound polynucleotides are separated from the bound species. The analyte polynucleotide is optionally displaced from the solid phase, then amplified by PCR. The PCR primers each have a polynucleotide region capable of hybridizing to a region of the analyte polynucleotide, and at least one of the primers further has an additional binding partner capable of binding a solid-phase binding partner. The amplified product is then separated from the reaction mixture by specific binding between the binding partners, and the amplified product is detected.Other References
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