Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
April 6, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesDiagnostic reagent Specific DNA probes in diagnostic microbiology Nucleic acid hybridization assay employing antibodies to intercalation complexes Method of detecting and characterizing a nucleic acid or reactant for the application of this method Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same Labeled DNA Monoclonal antibodies for DNA-RNA hybrid complexes and their uses Hybridization assay employing labeled probe and anti-hybrid Patent #: 4743535 InventorAssigneeApplicationNo. 188114 filed on 04/25/1988US Classes:435/6, Involving nucleic acid435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/803, PHYSICAL RECOVERY METHODS (E.G., CHROMATOGRAPHY, GRINDING)435/810, PACKAGED DEVICE OR KIT436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/508, Antinuclear (e.g., DNA, etc.)436/512, INVOLVING ANTIBODY FRAGMENTS436/808, Automated or kit530/387.1, Immunoglobulin, antibody, or fragment thereof, other than immunoglobulin antibody, or fragment thereof that is conjugated or absorbed536/24.32Probes for detection of microbial nucleotide sequencesExaminersPrimary: Yarbrough, Amelia B.Assistant: Marschel, Ardin H. Attorney, Agent or FirmForeign Patent References
International ClassC12Q 001/68AbstractA nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.Other References
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