Wholly microfabricated biosensors and process for the manufacture and use thereof
Patent 5200051 Issued on April 6, 1993. Estimated Expiration Date: April 6, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
204/403.07, For blocking passage of macromolecules (molecular weight greater than or equal to 8,000)204/403.09, Enzyme included in apparatus204/403.1, Enzyme included in apparatus204/403.11, Glucose oxidase204/403.13, And microelectrode (i.e., at least one electrode dimension is less than 500 microns)204/415, Selectively permeable membrane205/778, And using semipermeable membrane205/782.5, Using semipermeable membrane257/253, Chemical (e.g., ISFET, CHEMFET)422/930, Drop counters or drop formers [B01L 3/02D]435/287.9Including a coated reagent or sample layer
An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.
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April 6, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
