Methods of making fluorescent microspheres
March 16, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3853987 Cell specific, variable density, polymer microspheres Cell specific, variable density, polymer microspheres Immunological reagent employing radioactive and other tracers Preparation of small bio-compatible microspheres Attachment of proteins to inert particles Particulate support material Protein specific polymeric immunomicrospheres Protein specific fluorescent microspheres for labelling a protein Microsphere coated substrate containing reactive aldehyde groups InventorApplicationNo. 433677 filed on 11/08/1989US Classes:427/213.31, With post-treatment of encapsulant or encapsulating material (e.g., further coating, hardening, etc.)252/301.35, Synthetic resin containing428/407, Including synthetic resin or polymer436/532, Antigen or antibody attached to a carrier via bridging agent436/533, Carrier is water suspendible particles (e.g., latex, etc.)436/546, Fluorescent label436/800, FLUORESCENT DYES (E.G., RHODAMINE, ETC.)525/54.1, Containing chemically combined protein or biologically active polypeptide530/402, Chemical modification or the reaction product thereof, e.g., covalent attachment or coupling, etc.530/816Attached to the carrier via a bridging agentExaminersPrimary: Stoll, Robert L.Assistant: Covert, John M. Attorney, Agent or FirmForeign Patent References
International ClassesG01N 033/547C08F 008/10 AbstractHighly fluorescent latex microspheres have a diameter of less than five hundred angstroms and have more than five thousand fluorescent markers per sphere. The microspheres are prepared by reacting an acrylic latex bead with a diamine and a fluorescent amine at elevated pH. A protein such as avidin or an immunoglobulin may then be conjugated to the diamine. A single fluorescent microsphere is visible using standard fluorescent microscopy. Therefore the microspheres may be utilized not only to visualize cell surface anitgens but also DNA encoding for single genes, by means of a biotinylated DNA probe.Other References
Field of SearchSolid encapsulation process utilizing an emulsion or dispersion to form a solid-walled microcapsule (includes liposome)With post-treatment of encapsulant or encapsulating material (e.g., further coating, hardening, etc.) Solid-walled microcapsule formed by in situ polymerization Microcapsule with solid core (includes liposome) Carrier is water suspendible particles (e.g., latex, etc.) Carrier is water suspendible particles Containing chemically combined protein or biologically active polypeptide Solid polymer treated by stepwise reaction with naturally occurring alpha or beta amino acid or a material which contains a residue of said amino acid, e.g., a functionally protected amino acid, etc. INORGANIC LUMINESCENT COMPOSITIONS WITH ORGANIC NONLUMINESCENT MATERIAL Synthetic resin containing |
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