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US Patent 5188642 - Glyphosate-resistant plants

US Patent Issued on February 23, 1993
Estimated Patent Expiration Date: Icon_subject February 23, 2010Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
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Abstract

This invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.

Inventors

Application

No. 478794 filed on 02/12/1990

US Classes:

47/58.1R, PROCESS435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/69.7, Fusion proteins or polypeptides435/69.8, Signal sequence (e.g., beta-galactosidase, etc.)435/70.1, Using tissue cell culture to make a protein or polypeptide435/183, ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES504/117, Micro-organisms or from micro-organisms (e.g., fermentates, fungi, bacteria, viruses, etc.)504/195, Hetero ring containing504/197, Plural ring nitrogens in the hetero ring504/205, Additional -C(=X)-, or additional nitrogen attached indirectly to the phosphorus by acyclic nonionic bonding504/206, Containing -C(=X)X-, wherein the X`s are the same or diverse chalcogens (e.g., N-phosphonomethylglycines, etc.)800/300, Herbicide resistant plant which is transgenic or mutant800/300.1The plant is maize

Field of Search

435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/183, ENZYME (E.G., LIGASES (6. ), ETC.), PROENZYME; COMPOSITIONS THEREOF; PROCESS FOR PREPARING, ACTIVATING, INHIBITING, SEPARATING, OR PURIFYING ENZYMES435/69.7, Fusion proteins or polypeptides435/69.8, Signal sequence (e.g., beta-galactosidase, etc.)435/70.1Using tissue cell culture to make a protein or polypeptide

Examiners

Primary: Fox, David T.

Attorney, Agent or Firm

US Patent References

4940835Glyphosate-resistant plants
Issued on: 07/10/1990
Inventor: Shah, et al.

International Classes

A01H 001/00
A01H 005/10
C12P 021/00
C12P 021/04
C12N 015/00
C12N 009/00
A01N 057/00

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