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Plant proteins, promoters, coding sequences and use

Patent 5187267 Issued on February 16, 1993. Estimated Expiration Date: Icon_subject June 19, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventors

Assignee

Application

No. 541883 filed on 06/19/1990

US Classes:

800/298, Higher plant, seedling, plant seed, or plant part (i.e., angiosperms or gymnosperms)435/414, Tobacco cell or cell line, per se435/419, Plant cell or cell line, per se, contains exogenous or foreign nucleic acid536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/23.6, Encodes a plant polypeptide536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)800/317, Solanaceae (e.g., eggplant, etc.)800/317.3Tobacco

Examiners

Primary: Fox, David T.

Foreign Patent References

  • 330479 EP. 08/13/1989

International Classes

C07H 015/12
A01H 001/04
C12N 015/00
C12N 005/00

Abstract

Novel nucleic acid sequences, constructs employing such sequences, transgenic plant cells and plants are provided employing sequences associated with tomato heat shock protein 80.5 ("hsp80"). Nucleic acid sequences obtainable from the use of hsp80 as a probe in other plant species are also disclosed. In particular, the 5' noncoding region of tomato hsp80 or other plant promoters obtainable therefrom, especially to provide differentially specific initiation of transcription of a desired nucleic acid sequence of interest. Also provided are putative sequences believed to represent Scaffold Attachment Regions (SAR) of the plant hsp80 gene.

Other References

  • Smith et al. 1988. Nature 334:724-726
  • Schoffl et al. 1986. Phil. Trans. R. Soc. Lond. 314B:453-468
  • Schoffl et al. 1982. J. Mol. Appl. Genet. 1:301-314
  • Nover et al. 1984. Eur. J. Biochem. 139(2):303-313
  • Medford et al. 1989. The Plant Cell 1(4):403-413
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  • Hackett and Lis, "Localization of the hsp83 Transcript Within a 3292 Nucleotide Sequence From the 63B Heat Shock Locus of D. melanogaster," Nucl. Acids Res. (1983) 11:7011-7030
  • Czarnecka et al., "Comparative Analysis of Physical Stress Responses in Soybean Seedlings Using Cloned Heat Shock cDNAs," Plant Mol. Biol. (1984) 3:45-68
  • Heikkila et al., "Induction of Heat Shock Protein Messenger RNA in Maize Mesocotyls by Water Stress, Abscisic Acid, and Wounding" Plant Physiol. (1984) 76:270-274
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  • Lindquist, "The Heat-Shock Response," Ann. Rev. Biochem. (1986) 55:1151-1191
  • Kulomaa et al., "Amino Acid Sequence of a Chicken Heat Shock Protein Derived from the Complementary DNA Nucleotide Sequence," Biochemistry (1986) 25:6244-6251
  • Sargan et al., "hsp80, a Novel Heat Shock Inducible Protein of Chicken," Biochemistry (1986) 25:6252-6258
  • Czarnecka et al., "DNA Sequence and Transcript Mapping of a Soybean Gene Encoding a Small Heat Shock Protein," Proc. Nat. Acad. Sci. (1985) 82:3726-3730
  • Gurley et al., "Upstream Sequences Required for Efficient Expression of a Soybean Heat Shock Gene," Mol. Cell Biol. (1986) 6:559-565
  • Rochester et al., "The Structure and Expression of Maize Genes Encoding the Major Heat Shock Protein, hsp70," EMBO J. (1986) 5:451-458
  • Ananthan et al., "Abnormal Proteins Serve as Eukaryotic Stress Signals and Trigger the Activation of Heat Shock Genes," Science (1986) 232:522-524
  • Lindquist and Craig, "The Heat-Shock Proteins," Ann. Rev. Genet. (1988) 22:631-67
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