Process for transforming gramineae and the products thereof

Patent 5187073 Issued on February 16, 1993. Estimated Expiration Date:

February 16, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Inventors

Assignee

The University of Toledo

Application

No. 436187 filed on 11/13/1989

US Classes:

800/294, Via Agrobacterium435/252.2, Rhizobium or agrobacterium435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)

Examiners

Primary: Fox, David T.

Attorney, Agent or Firm

Marshall & Melhorn

International Classes

C12N 015/00
C12R 001/41

Abstract

A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment of T-DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling infected with vir+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir+ Agrobacterium tumefaciens and transformed Gramineae derived from seedlings infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.

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