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US Patent 5177010 - Process for transforming corn and the products thereof

US Patent Issued on January 5, 1993
Estimated Patent Expiration Date: Icon_subject September 5, 2010Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
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Abstract

A method of producing transformed corn comprising making a wound in a corn seedling in an area of the corn seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed corn pollen grain, a corn pollen grain produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a corn pollen grain produced by a plant grown from a seedling infected with vir° A. tumefaciens containing a vector comprising genetrically-engineered T-DNA, a corn pollen grain whose cells contain a segment of T-DNA, and corn derived from each of these pollen grains. There are also provided a transformed corn plant, a transformed corn plant derived from a corn seedling infected with vir+ Agrobacterium tumefaciens, a transformed corn plant derived from a corn seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, and a corn plant whose cells contain a segment of T-DNA. Finally, there are provided transformed corn derived from a corn seeding infected with vir+ Agrobacterium tumefaciens, and transformed corn derived from a corn seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.

Other References

  • Hernalsteens et al. 1984, EMBO J 3(13):3039-3041
  • Hooykaas-Van Slogteren et al. 1984, Nature 311:763-764
  • Lorz et al. 1985, Mol. Gen. Genet. 199:178-182
  • Potrykus et al. 1985, Mol. Gen. Genet. 199:183-188
  • de la Pena et al. 1981, Chromosoma 83(2):241-248
  • Korohoda et al. 1979, Z. Phanzenphysiol. 94:95-9

Inventors

Assignee

Application

No. 579354 filed on 09/05/1990

US Classes:

800/294, Via Agrobacterium435/252.2, Rhizobium or agrobacterium435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)

Field of Search

435/252.2, Rhizobium or agrobacterium435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)

Examiners

Primary: Fox, David T.

Attorney, Agent or Firm

International Classes

C12N 015/00
C12R 001/41
A01H 001/04

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