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AbstractA method of producing transformed corn comprising making a wound in a corn seedling in an area of the corn seedling containing rapidly dividing cells and inoculating the wound with vir+ Agrobacterium tumefaciens. Also, this same method wherein the vir+ A. tumefaciens contains a vector comprising genetically-engineered T-DNA. There are further provided a transformed corn pollen grain, a corn pollen grain produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a corn pollen grain produced by a plant grown from a seedling infected with vir° A. tumefaciens containing a vector comprising genetrically-engineered T-DNA, a corn pollen grain whose cells contain a segment of T-DNA, and corn derived from each of these pollen grains. There are also provided a transformed corn plant, a transformed corn plant derived from a corn seedling infected with vir+ Agrobacterium tumefaciens, a transformed corn plant derived from a corn seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA, and a corn plant whose cells contain a segment of T-DNA. Finally, there are provided transformed corn derived from a corn seeding infected with vir+ Agrobacterium tumefaciens, and transformed corn derived from a corn seedling infected with vir+ A. tumefaciens containing a vector comprising genetically-engineered T-DNA.Other References
| InventorsAssigneeApplicationNo. 579354 filed on 09/05/1990US Classes:800/294, Via Agrobacterium435/252.2, Rhizobium or agrobacterium435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)Field of Search435/252.2, Rhizobium or agrobacterium435/252.3, Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)435/320.1VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)ExaminersPrimary: Fox, David T.Attorney, Agent or FirmInternational ClassesC12N 015/00C12R 001/41 A01H 001/04 |