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System and method for improving sample concentration in capillary electrophoresis

Patent 5116471 Issued on May 26, 1992. Estimated Expiration Date: Icon_subject October 4, 2011. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventors

Application

No. 771575 filed on 10/04/1991

US Classes:

204/453, With injection204/604With injector

Examiners

Primary: Niebling, John F.
Assistant: Starsiak, John S. Jr.

International Classes

G01N 027/26
B01D 057/02

Abstract

The method for improving sample concentration in capillary electrophoresis by extracting the sample buffer from the capillary column using the electro-osmotic flow while the sample components are stacked into the support buffer utilizes a system for this technique. The system has a separation column 11, sample introduction means 16 for injecting the sample solution, an injection detector means 20 for detecting the volume of injected sample solution, a separation detector means 21 for detecting the sample components, power supply means 17 for applying a high voltage along the separation column 11 for reversing the direction of the electro-osmotic flow thereby causing extraction of the sample buffer from the separation column and afterward providing the separation sample into its components.

Other References

  • Ruedi Aebersold & Hamish D. Morrison "Analysis of dilute peptide samples by capillary zone electrophoresis" Journal of Chromatography 516 (1990) 79-88
  • Henk H. Lauer et al. "Analytical Aspects of an Automated Capillary Electrophoresis System" LC-GC vol. 6, No. 1 (1990) 34-46
  • F. E. B. Mikkers, F. M. Everaerts, & Th. P. E. M. Verheggen "High-Performance Zone Electrophoresis" Journal of Chromatography 169 (1979) 11-20
  • Dean S. Burgi & Ring-Ling Chien "Optimization in Sample Stacking for High-Performance Capillary Electrophoresis" Analytical Chemistry, vol. 63, No. 18 (1991) 2042-2047
  • Moring, Stephen E., "Analytical Aspects of an Automated Capillary Electrophoresis System," LC-GC, vol. 8, No. 1 (1990) 34-46
  • Jorgenson, James W. et al. "Capillary Zone Electrophoresis", Science, vol. 222, (1983), pp. 266-272
  • Nielen, M. W. F., "Impact of Experimental Parameters on the Resolution of Positional Isomers of Aminobenzoic Acid in Capillary Zone Electrophoresis," Journal of Chromatography, 542 (1991) 173-18
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