Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use
Patent 5094945 Issued on March 10, 1992. Estimated Expiration Date: 
March 10, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
March 10, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesInhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthase Patent #: 4971908 InventorAssigneeApplicationNo. 238209 filed on 08/30/1988US Classes:435/6, Involving nucleic acid435/193, Transferase other than ribonuclease (2.)435/444, By use of alkylating agent (e.g., nitrosoguanidine, etc.)435/481, Plasmid or episome contains a gene which complements a nutritional deficiency mutation536/23.2, Encodes an enzyme536/23.7Encodes a microbial polypeptideExaminersPrimary: Schwartz, Richard A.Assistant: Nolan, S. L. Attorney, Agent or FirmInternational ClassesC12N 009/10C12N 015/54 C12N 015/00 AbstractEnhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3-phosphoshikimate synthase (EC: 2.5.1.19) (ES-3-P synthase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the structural gene into a sensitive host, as well as providing a method of producing the enzyme.The E. coli strain C600 (pPMG1) has been deposited at the A.T.C.C. on December 14, 1982 and given A.T.C.C. Accession No. 39256.Other References
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