Laser excited confocal microscope fluorescence scanner and method

Patent 5091652 Issued on February 25, 1992. Estimated Expiration Date:

June 1, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Fluorescent microanalytical system and method for detecting and identifying organic materials
Patent #: 4087685
Issued on: 05/02/1978
Inventor: Froot

Fluorescence determining microscope utilizing laser light
Patent #: 4284897
Issued on: 08/18/1981
Inventor: Sawamura , et al.

Apparatus for investigation of fluorescence characteristics of microscopic objects
Patent #: 4354114
Issued on: 10/12/1982
Inventor: Karnaukhov , et al.

Two-dimensional scanning photo-electric microscope
Patent #: 4734578
Issued on: 03/29/1988
Inventor: Horikawa

Fluorescence microscopy
Patent #: 4791310
Issued on: 12/13/1988
Inventor: Honig , et al.

Scanning optical microscope
Patent #: 4893008
Issued on: 01/09/1990
Inventor: Horikawa

Heterodyne system and method for sensing a target substance Patent #: 5022757
Issued on: 06/11/1991
Inventor: Modell

Inventors

Application

No. 531900 filed on 06/01/1990

US Classes:

250/458.1, LUMINOPHOR IRRADIATION204/612, With detailed detection system (e.g., including a light source and a camera, etc.)250/459.1, Methods250/461.1, With ultraviolet source250/461.2Biological cell identification

Examiners

Primary: Hannaher, Constantine

Attorney, Agent or Firm

Flehr, Hohbach, Test, Albritton & Herbert

Foreign Patent References

WO86/05587 WO 09/13/1986

International Class

G01N 021/64

Abstract

A fluorescent scanner for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier including a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from said volume to provide a display of the separated sample.

Other References

Mathies, R. A. and Stryer, L. (1986), Applications of Fluorescence in the Biomedical Sciences, Eds. Taylor, D. L., Waggoner, A.SA., Lanni, F. Murphy, R. F., and Birge, R. (Alan R. Liss, Inc., New York) pp. 129-140
Nguyen, D. C.; Keller R. A.; Jett, J. H.; Martin, J. C. (1987), Detection of Single Molecules of Phycoerythrin in Hydrodynamically Focused Flows by Laser-Induced Fluorescence, Anal. Chem. 59, 2158-2161
Peck, K.; Stryer, L.; Glazer, A. N.; Mathies, R. A. (1989) Single molecule fluorescence detection: Autocorrelation criterion and experimental realization with phycoerythrin Proc. Natl. Acad. Sci. U.S.A., 86, 4087-4091
Mathies, R. A.; Peck, K.; Stryer, L. (1990), Optimization of High-sensitivity Fluorescence Detection, submitted for publication
Glaser, A. N.; Peck, K.; Mathies, R. A. (1990) A stable double-stranded DNA--ethidium homodimer complex: Application to picogram fluorescence detection of DNA in agarose gels, submitted for publication, Proc. Nat. Acad. Sci. U.S.A
Ansorge, W.; Rosenthal, A.; Sproat, B.; Schwager, C.; Stegemann, J.; & Voss, H. (1988) Non-radioactive automated sequencing of oligonucleotides by chemical degradation, Nucleic Acids Research vol. 16, 2203-2207
Smith, L. M.; Sanders, J. Z.; Kaiser, R. J.; Hughes, P.; Dodd, C.; Connell, C. R.; Heiner, C.; Kent, S. B. H. & Hood, L. E. (1986), Fluorescence detection in automated DNA sequence analysis, Nature, vol. 321, 674-679
Sanger, F.; Nicklen, S.; & Coulson, A. R. (1977), DNA sequencing with chain-terminating inhibitors, Proc. Natl. Acad. Sci., vol. 74, No. 12, pp. 5463-546