Apparatus for isotachophoretical separation
Horizontal electrophoresis cell for rapid assembly
Laser-excitation fluorescence detection electrokinetic separation
Integrated temperature control/alignment system for high performance capillary electrophoretic apparatus
Apparatus for capillary electrophoresis Patent #: 4985129
ApplicationNo. 399600 filed on 08/28/1989
US Classes:204/603With detailed detection system (e.g., including a light source and a camera, etc.)
ExaminersPrimary: Niebling, John F.
Assistant: Starsiak, John S. Jr.
Attorney, Agent or Firm
Foreign Patent References
International ClassesG01N 027/26
AbstractAn integrated temperature control/alignment system for use with a high performance capillary electrophoretic apparatus comprises a complementary pair of capillary column mounting plates formed from an electrically insulative, high thermal conductivity material for mounting a capillary column. The first mounting plate includes a well having a predetermined circumference, first and second grooves contiguous with the well and ports formed in the grooves and extending through the plate. The second mounting plate includes a plug configured for insertion in the well and ports aligned with the ports of the first mounting plate. Respective ends of the capillary column are seated and locked in the grooves to preposition the capillary column with respect to the ports. Capillary columns of varying lengths may be mounted within the mounting plates by coiling the intermediate portion thereof within the well to increase or decrease the working length of the column in predetermined increments, depending upon the particular application. Optical coupling elements are disposed in the ports to couple sampling and reference radiation to and from the mounted capillary column. The temperature of substantially the entire capillary column is controlled or regulated by at least one thermopile disposed in thermal contact with one of the mounting plates. A thermistor may be thermally coupled to the capillary column or one of the mounting plates to provide feedback signals to operate the thermopile to maintain the capillary column at a predetermined electrophoretic operating temperature.