Patent 5082767 Issued on January 21, 1992. Estimated Expiration Date: February 27, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
435/6, Involving nucleic acid435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/91.5, Acellular preparation of polynucleotide436/501, BIOSPECIFIC LIGAND BINDING ASSAY536/23.1, DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)536/24.1Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)
A method for determining the pattern of nonrandom codon pair usage of an organism, comprising the steps of obtaining nucleotide sequence data for the organism, determining from the data the number of codons represented in at least a portion of the sequence and the frequency of usage of at least some codons in the portion, determining from the frequency the expected number of occurrences of at least some codon pairs, if they are paired in a random manner, and comparing the expected number with the actual number of occurrences to determine relative codon pairing preferences. The codon pairings of organisms are highly nonrandom, and differ from organism to organism. This information is used to construct and express altered or synthetic genes having desired levels of translational efficiency, to determine which regions in a genome are protein coding regions, to introduce translational pause sites into heterologous genes, and to ascertain relationship or ancestral origin of nucleotide sequences.
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February 27, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
