Activation of macrophage tumoricidal activity by granulocyte-macrophage colony stimulating factor

Patent 5078996 Issued on January 7, 1992. Estimated Expiration Date:

January 7, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

Method for producing substance capable of stimulating differentiation and proliferation of human granulopoietic stem cells
Patent #: 4342828
Issued on: 08/03/1982
Inventor: Takaku , et al.

Unique T-lymphocyte line and products derived therefrom
Patent #: 4438032
Issued on: 03/20/1984
Inventor: Golde , et al.

Human cultured cell line Patent #: 4675291
Issued on: 06/23/1987
Inventor: Yamamura , et al.

Inventors

Assignee

Immunex Corporation

Application

No. 888995 filed on 07/31/1986

US Classes:

424/85.1, LYMPHOKINE424/93.71, Leukocyte424/534, Leucocyte435/69.5, Lymphokines or monokines514/2, Peptide containing (e.g., protein, peptones, fibrinogen, etc.) DOAI514/8, Glycoprotein (carbohydrate containing)514/21, Produced by or extracted from animal tissue530/350, PROTEINS, I.E., MORE THAN 100 AMINO ACID RESIDUES530/351, Lymphokines, e.g., interferons, interlukins, etc.530/828, Cancer536/23.5, Encodes an animal polypeptide536/23.51Hormone

Examiners

Primary: Kepplinger, Esther M.
Assistant: Kushan, Jeff

Attorney, Agent or Firm

Nagae; Jerald A., Wight; Christopher L., Hallquist; Scott G.

Foreign Patent References

8504188 WO. 09/22/1985

International Classes

A61K 045/05
A61K 037/66

Abstract

Macrophages and precursor monocytes are activated to exhibit tumoricidal activity by stimulation solely with granulocyte-macrophage colony stimulating factor. A patient suffering from tumors can be treated by direct administration of therapeutically effective quantities of activated granulocyte-macrophage colony stimulating factor. Homogeneous granulocyte-macrophage colony stimulating factor for use in activating macrophages and monocyte precursors is prepared by recombinant DNA techniques. The gene coding for granylocyte-macrophage colony stimulating factor is isolated and then recombinant protein product expressed in an appropriate expression system. The granulocyte-macrophage colony stimulating factor recovered from the expression system is purified to homogeneity by reverse phase high-performance liquid chromatography.

Other References

Binz et al, J. Exp. Med., 147, 63-76, (1978)
Berendt et al, J. Exp. Med., 151, 69, (1980)
Nepom et al, Experentia, 39(3), 235-242, (1983)
Flood et al., J. Exp. Med.; 154, 275-290, (1981)
Flood et al, PNAS, 77(3), 2209-2213, (Apr. 1980)
Stevenson et al, J. Immunol., 130(2), 970, (1983).
Den Otter, Lymphokines, 3, p. 389, (1981)
Moore et al, Lymplokines, 3, 119, (1981)
Grabstein et al, Science, 232, 506-508, (Apr. 1986)
Nathan et al, J. Exp. Med., 160, 600-605, (1984)
Handman, J. Imm., 122(3), 1134, (1979)
Nelson, in "Phagocytes & Cell. Immunity", (Gadebusch, Ed), CRC Press (1979)
Lang et al, Cell, 51, 675-686, (Nov. 1987)
Adams et al, Ann. Rev. Imm., 2: 283-318, (1984)
Arnaut et al, J. Clin. Invest., 78, 597-60, 1986
Burgess et al, Blood, 56 (6), 947, (1980)
Lopez et al, J. Immunol., 131(6), (Dec. 1983), pp. 2983-2988
Clark et al, Science, 236, 1229-1237, (Jun. 1987)
Weishart et al, Nature, 314, 361-364, (Mar. 1985)
Nicola et al, Blood, 54(3), 614, (1979)
Handman et al, J. Immunol., 122(3), 1134-1137, (1979)
Wing et al, J. Clin. Investigation, 69, 270-276, 1982
Ralph et al, Chem. Abs., 98:158918p, 1983
Meltzer, Lymphokines, vol. 3, pp. 319-343, 1981
Moore et al, Lymphokines, vol. 3, 119-148, 1981
Gough et al, Nature, 309, pp. 763-767, Jun. 1984
Moore et al, J. Immunol., 125(3), 1302-1305, 1980
Cameron et al., "Cytotoxicity of Human Macrophages for Tumor Cells", J. Clin. Invest. 63:977 (1979)
Mantovani et al., "Augmentation of Tumoricidal Activity of Human Monocytes and Macrophages by Lumphokines", Int. J. Cancer 25:691 (1980)
Kleinerman et al., "Tumoricidal Activity of Human Monocytes Activated In Vitro by Free and Liposome-encapsulated Human Lymphokines", J. Clin. Invest. 72:304 (1983)
Roberts and Vasil, J. Interferon Res. 2:519 (1982)
Svedersky et al., "Biological and Antigenic Similarities of Murine Interferon and Macrophage-Activating Factor", J. Exp. Med. 159:812 (1984)
Onozaki et al., "Role of Interleukin 1 in Promoting Human Monocyte-Mediated Tumor Cytotoxicity", J. Immunol., 135:314 (1985)
Sone et al., "In Vitro Generation of Tumoricidal Properties in Human Alveolar Macrophages Following Interaction with Endotoxin", Cancer Res. 42:2227 (1982)
Pick et al., "Intracellular Mediation of Lymphokine Action: Minicry of Migration Inhibitory Factor (MIF) Action by Phorbol Myristate (PMA) and the Ionophore A23187", Ann. N.Y. Acad. Sci. 332:378 (1979)
Hand et al., "Requirement for Magnesium Influx in Activation of Alveolar Macrophages Mediated by Ionophore A23187", Nature (London) 265:543 (1979)
Schultz et al., J. Interfon Res. 2:459 (1982