Digital biosensors and method of using same
Patent 5074977 Issued on December 24, 1991. Estimated Expiration Date: 
October 23, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
October 23, 2010. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3831432 Selective chemical sensitive FET transducers Integrated array of electrochemical sensors Field effect transistor for detection of biological reactions Analytical device having semiconductive polyacetylene element associated with analyte-binding substance Electrochemical measuring apparatus provided with an enzyme electrode Lipid membrane electroanalytical elements and method of analysis therewith Diagnostic device incorporating a biochemical ligand Apparatus for electrochemical measurements Device having photoresponsive electrode for determining analytes including ligands and antibodies InventorsAssigneeApplicationNo. 601940 filed on 10/23/1990US Classes:205/775, ELECTROLYTIC ANALYSIS OR TESTING (PROCESS AND ELECTROLYTE COMPOSITION)204/400, Analysis and testing204/403.06, With semipermeable membrane205/782, For oxygen or oxygen containing compound (except water)205/794.5, Using electrode containing precious metal or free carbon257/253, Chemical (e.g., ISFET, CHEMFET)435/4, MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP435/7.1, Involving antigen-antibody binding, specific binding protein assay or specific ligand-receptor binding assay435/817, ENZYME OR MICROBE ELECTRODE436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/806ELECTRICAL PROPERTY OR MAGNETIC PROPERTYExaminersPrimary: Tung, T.Attorney, Agent or FirmForeign Patent References
International ClassG01N 027/327AbstractA mearsuring instrument is disclosed having a reversibly selective binding protein immobilized upon the insulated-gate region of a field-effect transistor located on a sensor. With the sensor immersed in solution, the protein binds a select component of the solution to the gate producing an effect on a current flowing through the IGFET. A plurality of such binding protein-IGFET arrangements can be provided on the same sensor, including the same binding proteins having different binding coefficients KD or an array of proteins with different ligand specificity and/or affinity. Analysis of the IGFET's response to binding by a microprocessor allows, for example, the concentration of the component in solution to be determined. With a plurality of different binding proteins employed, the concentration of different components can be determined. Similarly, with binding proteins employed having different binding coefficients KD, the output of the sensor can be analyzed in either a digital or analog manner, or some combination of the two. To release the component from the protein, heat can be applied to the sensor through a resistive bank integrated into the sensor chip. Alternatively, the application of a reverse bias to the gate can be used to enhance release.Other References
Field of SearchAnalysis and testingIon-sensitive electrode Organic membrane MEASURING OR TESTING PROCESS INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITION OR TEST STRIP THEREFORE; PROCESSES OF FORMING SUCH COMPOSITION OR TEST STRIP ENZYME OR MICROBE ELECTRODE INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS ELECTRICAL PROPERTY OR MAGNETIC PROPERTY |
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