Serum-free culture medium for mammalian cells
Patent 5063157 Issued on November 5, 1991. Estimated Expiration Date: 
January 11, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
January 11, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSerum free cell culture medium Patent #: 4816401 InventorAssigneeApplicationNo. 295813 filed on 01/11/1989US Classes:435/387, Medium contains a polypeptide hormone435/6, Involving nucleic acid435/389, Culture medium contains a transferrin435/391, Culture medium contains an animal extract435/406, Contains a polypeptide hormone435/408Contains an animal extractExaminersPrimary: Weimar, Elizabeth C.Assistant: Wang, Gian P. Attorney, Agent or FirmForeign Patent References
International ClassesC12N 005/00C12N 005/02 C12Q 001/68 Foreign Application Priority Data1988-01-18 DEAbstractThe present invention provides a serum-free culture medium for mammalian cells, especially non-adherent cells, containing a base medium, transferrin, insulin and selenite, wherein it contains or consists of2 to 10 mg./liter transferrin,2 to 10 mg./liter insulin,1 to 10 g./liter peptone,10 to 500 mg./liter beta-D-xylopyranose substituted with phosphate, carboxyl and/or sulphate groups,0.1 to 0.5 mg./liter selenite and0.1 to 2 mg./liter biological polyamine.Other References
Field of SearchInvolving nucleic acid |
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