Process for controlling intracellular glycosylation of proteins
December 21, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesGene amplification in eukaryotic cells cDNA clones coding for polypeptides exhibiting multi-lineage cellular growth factor activity Vectors and methods for transformation of eucaryotic cells Process for producing human epidermal growth factor and analogs thereof CDNA clones coding for polypeptides exhibiting IGE binding factor activity High yield production of active Factor IX Vectors and compounds for expression of human protein C Patent #: 4775624 InventorsAssigneeApplicationNo. 288618 filed on 12/21/1988US Classes:435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/466, The polynucleotide is a shuttle vector or a transiently replicating hybrid vector536/23.2, Encodes an enzyme536/24.1Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)ExaminersPrimary: Schwartz, Richard A.Assistant: Nolan, S. L. Attorney, Agent or FirmInternational ClassesC12N 015/00C12N 015/56 C12N 015/79 AbstractA process for controlling the glycosylation of protein in a cell wherein the cell is genetically engineered to produce one or more enzymes which provide internal control of the cell's glycosylation mechanism. A Chinese hamster ovary (CHO) cell line is genetically engineered to produce a sialyltransferase. This supplemental sialyltransferase modifies the CHO glycosylation machinery to produce glycoproteins having carbohydrate structures which more closely resemble naturally occurring human glycoproteins.Other References
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