Method for the measurement of creatine or creatinine and reagents for these measurements
Patent 5047329 Issued on September 10, 1991. Estimated Expiration Date: 
September 10, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
September 10, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3806416 3806420 3907644 3912588 Creatinine amidohydrolase and creatine amidinohydrolase and process for producing them Analytical element and method for the determination of creatinine or creatine Patent #: 4812399 InventorAssigneeApplicationNo. 141043 filed on 01/05/1988US Classes:435/18, Involving hydrolase422/56, Having reagent in absorbent or bibulous substrate422/57, Having coated reagent422/58, In holder or container having special form435/25, Involving oxidoreductase435/28, Involving peroxidase435/805, TEST PAPERS436/175Digestion or removing interfering materialsExaminersPrimary: Nucker, Christine M.Assistant: Scheiner, Laurie Attorney, Agent or FirmInternational ClassC12Q 001/34Foreign Application Priority Data1987-01-23 JPAbstractA method for measuring creatine in a sample by the use of creatine amidinohydrolase which comprises decomposing the N-ethylglycine present in the sample enzymatically and thereafter reacting sarcosine oxidase upon the sample; and a reagent for use in the measurement of creatine comprising the first reagent and the second reagent, wherein the first reagent comprises a sarcosine oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 20 mM or below and catalase or comprises said sarcosine oxidase, a hydrogen donor oxidatively condensable with 4-aminoantipyrine and peroxidase and the second reagent comprises creatine amidinohydrolase, a sarcosin oxidase of which Km value to N-ethylglycine at pH 8, 37° C. is 50 mM or above, peroxidase and a color reagent for H2 O2.Other References
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