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US Patent 5035994 - Human stem cells and monoclonal antibodies

US Patent Issued on July 30, 1991
Estimated Patent Expiration Date: Icon_subject September 7, 2010Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
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Abstract

Monoclonal antibodies that recognize a stage-specific antigen on immature human marrow cells are provided. These antibodies are useful in methods of isolating cell suspensions from human blood and marrow that can be employed in bone marrow transplantation. Cell suspensions containing human pluripotent lympho-hematopoietic stem cells are also provided, as well as therapeutic methods employing the cell suspensions.

Other References

  • Perschel et al., "M7 Cluster Rept: CD34", ed. Knapp, at the Fourth Int'l Workshop and Conference on Human Leucocyte (1989), p. 817
  • Civin et al., "M7.1 Rept. on the CD34 Cluster Workshop", ed. Knapp, at the Fourth Int'l Workshop and Conference on Human Leucocyte (1989), p. 818
  • Lansdrop et al., "M7.2 CD34 Epitopes", ed. Knapp, at the Fourth Int'l Workshop and Conference on Human Leucocyte (1989), p. 826
  • Molgaard et al., "M7.3 Molecular Characterization of Human and Murine CD34", ed. Knapp, at the Fourth Int'l Workshop and Conference on Human Leucocyte (1989), p. 827
  • Civin et al., "Antigenic Analysis of Hematopoiesis III. A Hematopoietic Progenitor Cell Surface Antigen Defined by a Monoclonal Antibody Raised Against KG-1a Cells", J. Immunology 133:157-165 (1984)
  • Civin et al., "Cell Surface Antigens of Human Myeloid Cells", (1982) Exp. Hematol. 10:129 (abstract)
  • Civin et al., "Characterization of Four Monoclonal Antibodies Reactive with Human Cell Subsets", Blood, 60(5):95a (abstract)
  • Civin et al., "Cell Surface Antigens by Four Monoclonal Antibodies Raised Against KG-1a Cells", Hybridoma, 2:125a (abstract)
  • Brovall et al., "Identification and Partially Characterization of Two Human Potential Hematopoietic Differentiation Antigens", Exp. Hematol. 11:(Supp.) 199 (abstract)
  • Civin et al., "Diagnostic and Prognostic Utility of Cell Surface Markers in Acute Non-Lymphocytic Leukemia (ANLL)", Exp. Hematol. 11:(Supp.) 84
  • Strauss et al., "MY-10, A Human Hematopoietic Progenitor Cell Surface Antigen Identified by a Monoclonal Antibody", Exp. Hematol. 11:205 (abstract)
  • Strauss et al., "MY-10 Antigen is Lost at the CFU-E Stage During Erythroid Progenitor Cell Maturation", (1984) I.S.E.H. (Abstract)
  • Leary et al., "Single Cell Origin of Human Multilineage Colonies", (1984) I.S.E.H. (Abstract)
  • Bodger et al., "Surface Antigenic Determinants on Human Pluripotent and Unipotent Hematopoietic Progenitor Cells", (1983) Blood, 61:1006-1010
  • Bodger et al., "A Monoclonal Antibody Specific for Immature Human Hemopoietic Cells and T Lineage Cells", (1981) J. Immunology, 127:2269-2274
  • Nadler et al., "Diagnosis and Treatment of Human Leukemias and Lymphomas Utilizing Monoclonal Antibodies", Prog. Hematol., 12:187-225
  • Winchester et al., "Expression of Ia-Like Antigen Molecules on Human Granulocytes During Early Phases of Differentiation", Proc. Natl. Acad. Sci. U.S.A. 74:4012-4016 (1977)
  • Stashenko et al., "Characterization of a Human B Lymphocyte-Specific Antigen", J. Immunology, 125:1678-1685
  • Reinherz, et al., "Regulation of the Human Response--Inducer and Suppressor T-Lymphocyte Subsets in Human Beings", (1980) N. Engl. J. Med. 303:370
  • Ritz et al., "A Monoclonal Antibody to Human Acute Lymphoblastic Leukaemia Antigen", Nature 283:583-58

Inventor

Assignee

Application

No. 580337 filed on 09/07/1990

US Classes:

435/2, MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT435/7.21, Animal cell435/7.23, Tumor cell or cancer cell435/7.24, Leukocyte (e.g., lymphocyte, granulocyte, monocyte, etc.)436/548Monoclonal antibody

Field of Search

435/2, MAINTAINING BLOOD OR SPERM IN A PHYSIOLOGICALLY ACTIVE STATE OR COMPOSITIONS THEREOF OR THEREFOR OR METHODS OF IN VITRO BLOOD CELL SEPARATION OR TREATMENT436/548Monoclonal antibody

Examiners

Primary: Moskowitz, Margaret
Assistant: Cunningham, T.

Attorney, Agent or Firm

US Patent References

4364932, Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same
Issued on: 12/21/1982
Inventor: Kung ,   et al.
4364937, Monoclonal antibody to a human T cell antigen and methods of preparing same
Issued on: 12/21/1982
Inventor: Kung ,   et al.
4381292, Anti-human T-lymphocyte monoclonal antibody
Issued on: 04/26/1983
Inventor: Bieber ,   et al.
4381295, Monoclonal antibody to human helper T cells and methods of preparing same
Issued on: 04/26/1983
Inventor: Kung ,   et al.
4443427, Monoclonal antibody
Issued on: 04/17/1984
Inventor: Reinherz ,   et al.
4582797, Monoclonal antibodies specific for human hematopoietic cell surface glycoproteins
Issued on: 04/15/1986
Inventor: Trowbridge
4624925, Hybrid cell line for producing monoclonal antibody to a human early thymocyte antigens, antibody, and methods
Issued on: 11/25/1986
Inventor: Kung ,   et al.
4710457Monoclonal antibody for human hematopoietic glycoproteins and method
Issued on: 12/01/1987
Inventor: Dupont ,   et al.

International Classes

A01N 001/02
A61M 037/00
C12Q 001/00
G01N 033/53

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