Patent 5004863 Issued on April 2, 1991. Estimated Expiration Date: April 2, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
800/314, Cotton435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/427, Involving cotton cell or tissue435/430.1, Involving callus or embryonic stage435/469, Introduction via Agrobacterium800/278, METHOD OF INTRODUCING A POLYNUCLEOTIDE MOLECULE INTO OR REARRANGEMENT OF GENETIC MATERIAL WITHIN A PLANT OR PLANT PART800/288, Nonplant protein is expressed from the polynucleotide800/294, Via Agrobacterium800/300Herbicide resistant plant which is transgenic or mutant
A method is disclosed to achieve genetic transformation of cotton plants and lines. Immature cotton tissues are genetically transformed in vitro, by Agrobacterium-mediated genetic transformation. The resultant cotton tissues are subjected to a selection agent or agents to screen for transformants. The transformed cultures are then induced to commence somatic embryogenesis. One possible regime for regenerating such somatic embryos into whole cotton plants is disclosed.
Other References
De Block et al., 1984, EMBO J 3(8):1681-1689
Zutra, D. 1982, Plant Disease 66(12):1200-1201
Adang et al., 1985, Chem. Abstr. 103(9):#66080c
Barton et al., "Regeneration of Intact Tobacco Plants Containing Full Length Copies of Genetically Engineered T-DNA, and Transmission of T-DNA to R1 Progeny," Cell, vol. 32, pp. 1033-1043 (1983)
Beasley et al., "The Effects of Plant Growth Substances on In Vitro Fiber Development from Fertilized Cotton Ovules," Amer. J. Bot., vol. 60(2): pp. 130-139 (1973)
Bevan, "Binary Agrobacterium Vectors for Plant Transformation," Nucleic Acids Research, vol. 12:22, pp. 8711-8721 (1984)
Davidonis et al, "Plant Regeneration from Callus Tissue of Gossypium hirsutum L.", Plant Sc. Lettersvol. 32, pp. 89-93 (1983)
Davis et al., "In Vitro Culture of Callus Tissues and Cell Suspensions from Okra (Hibiscus esculentus L. ) and Cotton (Gossypium hirsutum L.)," In Vitro, vol. 9:6, pp. 395-398 (1974)
de Framond et al., "Mini-Ti: A New Vector Strategy for Plant Genetic Engineering," Bio/Technology, vol. 1, pp. 262-269 (May 1983)
Finer et al., "Initiation of Callus and Somatic Embryos from Explants of Mature Cotton (Gossypium klotzschianum Anderss), " Plant Cell Rep., vol. 3, pp. 41-43 (1984)
Firoozabady, et al., "Transformation of Cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and Regeneration of Transgenic Plants," Plant Molecular Biology 10:105-116 (1987)
Gamborg et al., "Nutrient Requirements of Suspension Cultures of Soybean Root Cells," Exp. Cell Res., vol. 50, pp. 151-158 (1968)
Gorman et al., "Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells," Mol. and Cell. Biol., vol. 2:9, pp. 1044-1051 (1982)
Herrera-Estrella et al., "Expression of Chimaeric Genes Transferred into Plant Cells Using a Ti-Plasmid-Derived Vector," Nature, vol. 303:19, pp. 209-213 (1983)
Hoekema et al., "A Binary Plant Vector Strategy Based on Separation of Vir- and T-Region of the Agrobacterium tumefaciens Ti-Plasmid," Nature, vol. 303, p. 179-180 (12 May 1983)
Linsmaier et al., "Organic Growth Factor Requirements of Tobacco Tissue Cultures," Physiologia Plantarum, vol. 18, pp. 100-127 (1965)
Mitten, "Somatic Embryogenesis in Gossypium hirsutum L., " Proc. of Beltwide Cotton Prod. Res. Conf., pp. 57-58 (Jan. 1985)
Murashige et al., "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures," Physiologia Plantarium, vol. 15, pp. 473-497 (1962)
Paszkowski et al., "Direct Gene Transfer to Plants," EMBO Jour., vol. 3:12, pp. 2717-2722 (1984)
Price et al., "Somatic Embryogenesis in Suspension Cultures of Gossypium klotzschianum Anderss," Planta, vol. 145, pp. 305-306 (1979)
Price et al., "Callus Cultures of Six Species of Cotton (Gossypium L.) on Defined Media," Plant Sci. Letters, vol. 10, pp. 115-119 (1977)
Rangan et al., "Somatic Enbryogenesis in Tissue Cultures of Gossypium hirsutum L.", In Vitro, vol. 20, p. 256 (1984)
Rani et al., "Establishment of Tissue Cultures of Cotton," Plant Science Letters, vol. 7, pp. 163-169 (1976)
Reiss et al., "A New Sensitive Method for Qualitative and Quantitative Assay of Neomycin Phosphortransferase in Crude Cell Extracts," Gene, vol. 30, pp. 211-218 (1984)
Robacker et al., "Somatic Embryogensis in Two Strains of Gossypium hirsutum, " Ann. Mtg. of the Amer. Soc. of Agronomy, p. 85, (Nov. 1984)
Stewart et al., "In-Ovulo Embryo Culture and Seedling Development of Cotton (Gossypium hirsutum, L.)," Planta, vol. 137, pp. 113-117 (1977)
Trolinder et al., "Somatic Embryogensis in Cell Suspension Cultures of Gossypium hirsutum," Proc. of Beltwide Cotton Prod. Res. Confs., p. 46 (Jan. 1985)
Umbeck et al., "Genetically Transformed Cotton (Gossypium hirsutum L.) Plants," Bio/Technologyvol. 5, pp. 263-266 (1987)
Zhou et al., "Introduction of Exogenous DNA into Cotton Embryos," Methods in Enzmology, vol. 101, pp. 433-481 (1983
April 2, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
