Covalent attachment of antibodies and antigens to solid phases using extended length heterobifunctional coupling agents
October 11, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent ReferencesSupported receptor and use thereof in an assay Antigenic modification of polypeptides Multiple-component binding assay system and method of making and using it Polyether polyamines as linking agents for particle reagents useful in immunoassays Process for labeling nucleic acids using psoralen derivatives Covalently-modified polyanionic bacterial polysaccharides, stable covalent conjugates of such polysaccharides and immunogenic proteins with bigeneric spacers, and methods of preparing such polysaccharides and conjugates and of confirming covalency Biotinylating agents Patent #: 4794082 InventorsApplicationNo. 254288 filed on 10/11/1988US Classes:435/176, Enzyme or microbial cell is immobilized on or in an inorganic carrier435/177, Enzyme or microbial cell is immobilized on or in an organic carrier435/181, Attached to the carrier via a bridging agent435/188, Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate435/207, Acting on beta-galatose-glycoside bond (e.g., beta-galactosidase, etc.)436/527, Glass or silica436/532, Antigen or antibody attached to a carrier via bridging agent530/391.1, Monoclonal or polyclonal antibody or immunoglobulin or fragment thereof that is conjugated or adsorbed (e.g., adsorbed to a solid support, etc.)530/405Nitrogen containing reactantExaminersPrimary: Moskowitz, MargaretAssistant: Kim, Harry C. Attorney, Agent or FirmForeign Patent References
International ClassesC12N 011/06C07K 017/06 AbstractNovel methods for covalent attachment of antibodies, antigens, or other molecules to solid phases using extended length heterobifunctional crosslinking reagents are disclosed. The resulting derivatized solid phases can be used in diagnostic assays.Other References
Field of SearchCARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOFAttached to the carrier via a bridging agent Enzyme or microbial cell is immobilized on or in an organic carrier Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate Enzyme or microbial cell is immobilized on or in an inorganic carrier Involving phosphatase Involving peroxidase Acting on beta-galatose-glycoside bond (e.g., beta-galactosidase, etc.) Nitrogen containing reactant Nitrogen attached directly or indirectly to the five-membered hetero ring by acyclic nonionic bonding Plural chalcogens bonded directly to ring carbons of each of the two nitrogen containing hetero rings (e.g., bis-succinimides, etc.) Glass or silica Antigen or antibody attached to a carrier via bridging agent Containing chemically combined protein or biologically active polypeptide |
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