High performance microcapillary gel electrophoresis

Patent 4997537 Issued on March 5, 1991. Estimated Expiration Date:

September 12, 2009. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

2748074

3616457

3691054

3728145

3783118

3876775

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Inventors

Assignee

Northeastern University

Application

No. 406080 filed on 09/12/1989

US Classes:

204/453, With injection204/455, Using gel-filled capillary204/605Gel filled

Examiners

Primary: Niebling, John F.
Assistant: Starsiak, John S. Jr.

Attorney, Agent or Firm

Weingarten, Schurgin, Gagnebin & Hayes

Foreign Patent References

0272925 EP. 06/13/1988
61-114115 JP 05/13/1986
63-210766 JP 09/13/1988
63-210767 JP 09/13/1988
63-210768 JP 09/13/1988
1233907 GB. 06/13/1971

International Classes

G01N 027/26
G01N 027/28
B01D 057/02

Abstract

A microcapillary column for high performance electrophoresis. A preferred column includes a microcapillary, a thin layer of coating material covalently bonded to the inner surface of the microcapillary wall, a thin layer of a hydrophilic polymer absorbed on the layer of coating material, and a gel comprising polyacrylamide polymerized in the tube, filling it. The gel-containing microcapillary is prepared by covalently bonding a layer of a suitable coating material to the inner surface of the microcapillary wall, applying a layer of hydrophilic polymer, and then causing a mixture of monomer with or without crosslinking agent, initiator, and polymerization catalyst to react in the bore of the microcapillary to form a polymeric matrix. In electrophoresis, the gel-containing microcapillary provides peak efficiencies in excess of 100,000 theoretical plates and in some instances over 1,000,000 theoretical plates within separation times of less than thirty minutes, permits trace level determinations of molecular weights, and permits electrophoretic operation at fields of 300 V/cm or higher, resulting in extremely high resolution separations.

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