Phycobiliprotein fluorescent conjugates
Fluorescent multiparameter particle analysis
Fluorescent energy transfer with phycobiliproteins Patent #: 4666862
ApplicationNo. 511834 filed on 04/13/1990
US Classes:435/6, Involving nucleic acid436/501, BIOSPECIFIC LIGAND BINDING ASSAY436/800, FLUORESCENT DYES (E.G., RHODAMINE, ETC.)536/24.3, Probes for detection of specific nucleotide sequences or primers for the synthesis of DNA or RNA536/24.31Probes for detection of animal nucleotide sequences
ExaminersPrimary: Yarbrough, Amelia B.
Attorney, Agent or Firm
Foreign Patent References
International ClassC12Q 001/68
AbstractFluorescent stokes shift probes for polynucleotide hybridization assays are designed to provide predetermined nucleotide base unit spacings between the donor and acceptor fluorophores. When the probes are hybridized to the target polynucleotide the fluorophores paired for non-radiative energy transfer are separated by 2 to 7 nucleotide base units. The fluorophores are attached to the DNA or RNA probes by linker arms having lengths of 4 to 30 Angstroms. Fluorescein is a preferred donor for use with a Texas Red acceptor.