U.S. patents available from 1976 to present.
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Methods for preparing sample nucleic acids for hybridization

Patent 4983523 Issued on January 8, 1991. Estimated Expiration Date: Icon_subject April 8, 2008. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Inventors

Assignee

Application

No. 179349 filed on 04/08/1988

US Classes:

435/173.7, Lytic effect produced (e.g., disruption of cell membrane for release of subcellular parts; e.g., nucleic acids, etc.)435/89, Nucleotide435/259, Lysis of micro-organism435/803PHYSICAL RECOVERY METHODS (E.G., CHROMATOGRAPHY, GRINDING)

Examiners

Primary: Lilling, Herbert J.

Attorney, Agent or Firm

International Classes

C12N 013/00
C12N 001/06
C12P 019/30

Abstract

A new method for releasing sample nucleic acids from cells, bacteria and viruses comprises non-invasively sonicating the sample contained within a sample container brought into physical contact with the vibrating element of a sonicator tuned to resonate at a frequency of 40 KHz or greater.

Other References

  • Derwent Abs. 87-313999/45 Gen. Probe AU8770404 (9-1987)
  • Derwent Abs. 83-769875/38 SU975020 (12-1982) Moskalenko et al
  • Derwent Abs. 81-60507D/34 DD148447 (5-1987) Shishkov et al
  • Biotech. Abs. 88-03296 DD243873 (3-1987) Akad
  • Biotech Abs. 86-06167 "Enzyme Microbe Tech" (1986)8,4,192-204, Chisti
  • Biotech. Abs. 82-04593 Biotechnol Lett(1982)4,653-54 Patel et al
  • Derwent Abst. 88-163415/24 EP-271448 (6-1988) Ringros
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