Patent ReferencesInhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use Patent #: 4769061 InventorsApplicationNo. 879814 filed on 07/07/1986US Classes:800/288, Nonplant protein is expressed from the polynucleotide435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/411, Tomato cell or cell line, per se435/414, Tobacco cell or cell line, per se435/415, Soybean cell or cell line, per se435/416, Sunflower cell or cell line, per se435/418, Plant cell or cell line, per se, is pest or herbicide resistant or pest lethal536/23.6, Encodes a plant polypeptide536/24.1, Non-coding sequences which control transcription or translation processes (e.g., promoters, operators, enhancers, ribosome binding sites, etc.)800/294, Via Agrobacterium800/300, Herbicide resistant plant which is transgenic or mutant800/300.1The plant is maizeExaminersPrimary: Warren, Charles F.Assistant: Fox, David T. Attorney, Agent or FirmForeign Patent References
International ClassesA01H 001/04C12N 015/00 C12N 005/00 C07H 015/12 AbstractThis invention involves a cloning or expression vector comprising a gene which encodes 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) polypeptide which, when expressed in a plant cell contains a chloroplast transit peptide which allows the polypeptide, or an enzymatically active portion thereof, to be transported from the cytoplasm of the plant cell into a chloroplast in the plant cell, and confers a substantial degree of glyphosate resistance upon the plant cell and plants regenerated therefrom.The EPSPS coding sequence may be ligated to a strong promoter, such as the 35S promoter from cauliflower mosaic virus, to create a chimeric gene. Such genes can be inserted into plant transformation vectors, and subsequently introduced into plant cells. Plant cells transformed using such genes and plants regenerated therefrom have been shown to exhibit a substantial degree of glyphosate resistance.Other References
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